{"data":{"id":"10.6084/m9.figshare.3824379","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3824379","prefix":"10.6084","suffix":"m9.figshare.3824379","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Mori, Masato","nameType":"Personal","givenName":"Masato","familyName":"Mori","affiliation":[],"nameIdentifiers":[]},{"name":"Motomu Hashimoto","affiliation":[],"nameIdentifiers":[]},{"name":"Matsuo, Takashi","nameType":"Personal","givenName":"Takashi","familyName":"Matsuo","affiliation":[],"nameIdentifiers":[]},{"name":"Fujii, Takao","nameType":"Personal","givenName":"Takao","familyName":"Fujii","affiliation":[],"nameIdentifiers":[]},{"name":"Moritoshi Furu","affiliation":[],"nameIdentifiers":[]},{"name":"Hiromu Ito","affiliation":[],"nameIdentifiers":[]},{"name":"Yoshitomi, Hiroyuki","nameType":"Personal","givenName":"Hiroyuki","familyName":"Yoshitomi","affiliation":[],"nameIdentifiers":[]},{"name":"Hirose, Jun","nameType":"Personal","givenName":"Jun","familyName":"Hirose","affiliation":[],"nameIdentifiers":[]},{"name":"Yoshinaga Ito","affiliation":[],"nameIdentifiers":[]},{"name":"Akizuki, Shuji","nameType":"Personal","givenName":"Shuji","familyName":"Akizuki","affiliation":[],"nameIdentifiers":[]},{"name":"Nakashima, Ran","nameType":"Personal","givenName":"Ran","familyName":"Nakashima","affiliation":[],"nameIdentifiers":[]},{"name":"Imura, Yoshitaka","nameType":"Personal","givenName":"Yoshitaka","familyName":"Imura","affiliation":[],"nameIdentifiers":[]},{"name":"Naoichiro Yukawa","affiliation":[],"nameIdentifiers":[]},{"name":"Yoshifuji, Hajime","nameType":"Personal","givenName":"Hajime","familyName":"Yoshifuji","affiliation":[],"nameIdentifiers":[]},{"name":"Ohmura, Koichiro","nameType":"Personal","givenName":"Koichiro","familyName":"Ohmura","affiliation":[],"nameIdentifiers":[]},{"name":"Tsuneyo Mimori","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Cell-contact-dependent activation of CD4\u003csup\u003e+\u003c/sup\u003e T cells by adhesion molecules on synovial fibroblasts"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2017,"subjects":[{"subject":"Biophysics"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"Hematology"}],"contributors":[],"dates":[{"date":"2016-09-13","dateType":"Created"},{"date":"2017-05-05","dateType":"Updated"},{"date":"2017","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/14397595.2016.1220353","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3499838 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eObjective\u003c/i\u003e: To determine how cell–cell contact with synovial fibroblasts (SF) influence on the proliferation and cytokine production of CD4\u003csup\u003e+ \u003c/sup\u003eT cells. \u003ci\u003eMethods\u003c/i\u003e: Naïve CD4\u003csup\u003e+ \u003c/sup\u003eT cells were cultured with SF from rheumatoid arthritis patients, stimulated by anti-CD3/28 antibody, and CD4\u003csup\u003e+ \u003c/sup\u003eT cell proliferation and IFN-γ/IL-17 production were analyzed. To study the role of adhesion molecules, cell contact was blocked by transwell plate or anti-intracellular adhesion molecule-1 (ICAM-1)/vascular cell adhesion molecule-1(VCAM-1) antibody. To study the direct role of adhesion molecules for CD4\u003csup\u003e+ \u003c/sup\u003eT cells, CD161\u003csup\u003e+ \u003c/sup\u003eor CD161\u003csup\u003e-\u003c/sup\u003e naïve CD4\u003csup\u003e+ \u003c/sup\u003eT cells were stimulated on plastic plates coated by recombinant ICAM-1 or VCAM-1, and the source of IFN-γ/IL-17 were analyzed. \u003ci\u003eResults\u003c/i\u003e: SF enhanced naïve CD4\u003csup\u003e+ \u003c/sup\u003eT cell proliferation and IFN-γ/IL-17 production in cell-contact and in part ICAM-1-/VCAM-1-dependent manner. Plate-coated ICAM-1 and VCAM-1 enhanced naïve CD4\u003csup\u003e+ \u003c/sup\u003eT cell proliferation and IFN-γ production, while VCAM-1 efficiently promoting IL-17 production. CD161\u003csup\u003e+ \u003c/sup\u003enaïve T cells upregulating LFA-1 and VLA-4 were the major source of IFN-γ/IL-17 upon interaction with ICAM-1/VCAM-1. \u003ci\u003eConclusion\u003c/i\u003e: CD4\u003csup\u003e+ \u003c/sup\u003eT cells rapidly expand and secrete IFN-γ/IL-17 upon cell-contact with SF via adhesion molecules. Interfering with ICAM-1-/VCAM-1 may be beneficial for inhibiting RA synovitis.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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