{"data":{"id":"10.6084/m9.figshare.3543293","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3543293","prefix":"10.6084","suffix":"m9.figshare.3543293","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Pritha Ghosh","affiliation":[],"nameIdentifiers":[]},{"name":"Grellscheid, Sushma Nagaraja","nameType":"Personal","givenName":"Sushma Nagaraja","familyName":"Grellscheid","affiliation":[],"nameIdentifiers":[]},{"name":"R. Sowdhamini","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"A tale of two paralogs: human Transformer2 proteins with differential RNA-binding affinities"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"Inorganic Chemistry"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-08-05","dateType":"Created"},{"date":"2016-08-05","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/07391102.2015.1100551","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["149269667 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The Transformer2 (Tra2) proteins in humans are homologues of the \u003ci\u003eDrosophila\u003c/i\u003e Tra2 protein. One of the two RNA-binding paralogs, Tra2β, has been very well-studied over the past decade, but not much is known about Tra2α. It was very recently shown that the two proteins demonstrate the phenomenon of paralog compensation. Here, we provide a structural basis for this genetic backup circuit, using molecular modelling and dynamics studies. We show that the two proteins display similar binding specificities, but differential affinities to a short GAA-rich RNA stretch. Starting from the 6-nucleotide RNA in the solution structure, close to 4000 virtual mutations were modelled on RNA and the domain–RNA interactions were studied after energy minimisation to convergence. Separately, another known 13-nucleotide stretch was docked and the domain–RNA interactions were observed through a 100-ns dynamics trajectory. We have also demonstrated the ‘compensatory’ mechanism at the level of domains in one of the domain repeat-containing RNA-binding proteins.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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