{"data":{"id":"10.6084/m9.figshare.3505454","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3505454","prefix":"10.6084","suffix":"m9.figshare.3505454","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Castelain, Mickaël","nameType":"Personal","givenName":"Mickaël","familyName":"Castelain","affiliation":[],"nameIdentifiers":[]},{"name":"Duviau, Marie-Pierre","nameType":"Personal","givenName":"Marie-Pierre","familyName":"Duviau","affiliation":[],"nameIdentifiers":[]},{"name":"Oxaran, Virginie","nameType":"Personal","givenName":"Virginie","familyName":"Oxaran","affiliation":[],"nameIdentifiers":[]},{"name":"Schmitz, Philippe","nameType":"Personal","givenName":"Philippe","familyName":"Schmitz","affiliation":[],"nameIdentifiers":[]},{"name":"Cocaign-Bousquet, Muriel","nameType":"Personal","givenName":"Muriel","familyName":"Cocaign-Bousquet","affiliation":[],"nameIdentifiers":[]},{"name":"Loubière, Pascal","nameType":"Personal","givenName":"Pascal","familyName":"Loubière","affiliation":[],"nameIdentifiers":[]},{"name":"Jean-Christophe Piard","affiliation":[],"nameIdentifiers":[]},{"name":"Mercier-Bonin, Muriel","nameType":"Personal","givenName":"Muriel","familyName":"Mercier-Bonin","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Oligomerized backbone pilin helps piliated \u003ci\u003eLactococcus lactis\u003c/i\u003e to withstand shear flow"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"Space Science"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Inorganic Chemistry"}],"contributors":[],"dates":[{"date":"2016-07-29","dateType":"Created"},{"date":"2016-08-19","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/08927014.2016.1213817","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["2560828 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The present work focuses on the role of pili present at the cell surface of \u003ci\u003eLactococcus lactis\u003c/i\u003e in bacterial adhesion to abiotic (hydrophobic polystyrene) and biotic (mucin-coated polystyrene) surfaces. Native pili-displaying strains and isogenic derivatives in which pilins or sortase C structural genes had been modified were used. Surface physico-chemistry, morphology and shear-flow-induced detachment of lactococcal cells were evaluated. The involvement of pili in \u003ci\u003eL. lactis\u003c/i\u003e adhesion was clearly demonstrated, irrespective of the surface characteristics (hydrophobic/hydrophilic, presence or not of specific binding sites). The accessory pilin, PilC, and the backbone pilin, PilB, were revealed to play a major role in adhesion, provided that the PilB was present in its polymerized form. Within the population fraction that remained attached to the surface under increasing shear flow, different association behaviors were observed, showing that pili could serve as anchoring sites thus hampering the effect of shear flow on cell orientation and detachment.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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