{"data":{"id":"10.6084/m9.figshare.3502025.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3502025.v1","prefix":"10.6084","suffix":"m9.figshare.3502025.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Singh, Alok Kumar","nameType":"Personal","givenName":"Alok Kumar","familyName":"Singh","affiliation":[],"nameIdentifiers":[]},{"name":"Pandey, Rajeev Kumar","nameType":"Personal","givenName":"Rajeev Kumar","familyName":"Pandey","affiliation":[],"nameIdentifiers":[]},{"name":"Shaha, Chandrima","nameType":"Personal","givenName":"Chandrima","familyName":"Shaha","affiliation":[],"nameIdentifiers":[]},{"name":"Rentala Madhubala","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"MicroRNA expression profiling of \u003ci\u003eLeishmania donovani\u003c/i\u003e-infected host cells uncovers the regulatory role of \u003ci\u003eMIR30A-3p\u003c/i\u003e in host autophagy"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-07-26","dateType":"Created"},{"date":"2016-07-26","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15548627.2016.1203500","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.3502025","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["196683 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eLeishmania\u003c/i\u003e is an obligate intracellular parasite that replicates inside phagolysosomes or parasitophorous vacuole (PV) of the monocyte/macrophage lineage. It reprograms macrophages and produces a metabolic state conducive to successful infection and multiplication. MicroRNAs (miRNAs), a class of small 22 to 24 nucleotide noncoding regulatory RNAs alter the gene expression and consequently proteome output by targeting mRNAs, may play a regulatory role in modulating host cell functions. In the present study, we demonstrate the novel regulatory role of host microRNA, \u003ci\u003eMIR30A-3p\u003c/i\u003e in modulation of host cell macroautophagy/autophagy after infection with \u003ci\u003eL. donovani\u003c/i\u003e. Our \u003ci\u003ein vitro\u003c/i\u003e studies showed that \u003ci\u003eMIR30A-3p\u003c/i\u003e expression was significantly enhanced after \u003ci\u003eL. donovani\u003c/i\u003e infection in a time-dependent manner. Transient transfection with a \u003ci\u003eMIR30A-3p\u003c/i\u003e inhibitor followed by \u003ci\u003eL. donovani\u003c/i\u003e infection promoted the autophagic response and decreased the intracellular parasite burden in both THP-1 cells and human monocyte-derived macrophages (HsMDM). BECN1/Beclin 1, the mammalian ortholog of yeast Vps30/Atg6, is a key autophagy-promoting protein that plays a key role in the regulation of cell death and survival. We report BECN1 dependent modulation of host cell autophagy in response to \u003ci\u003eL. donovani\u003c/i\u003e infection. Pretreatment of \u003ci\u003eL. donovani\u003c/i\u003e-infected macrophages with the \u003ci\u003eMIR30A-3p\u003c/i\u003e mimic decreased and with antagomir increased the expression of BECN1 protein. We demonstrate that \u003ci\u003eBECN1\u003c/i\u003e is a potential target of \u003ci\u003eMIR30A-3p\u003c/i\u003e and this miRNA negatively regulates \u003ci\u003eBECN1\u003c/i\u003e expression. Our present study for the first time reveals a novel role of \u003ci\u003eMIR30A-3p\u003c/i\u003e in regulating autophagy-mediated \u003ci\u003eL. donovani\u003c/i\u003e elimination by targeting \u003ci\u003eBECN1\u003c/i\u003e. The present study has significant impact in the treatment of visceral leishmaniasis.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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