{"data":{"id":"10.6084/m9.figshare.3492152.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3492152.v1","prefix":"10.6084","suffix":"m9.figshare.3492152.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Malik, Sadiya","nameType":"Personal","givenName":"Sadiya","familyName":"Malik","affiliation":[],"nameIdentifiers":[]},{"name":"Saito, Hiroko","nameType":"Personal","givenName":"Hiroko","familyName":"Saito","affiliation":[],"nameIdentifiers":[]},{"name":"Takaoka, Miho","nameType":"Personal","givenName":"Miho","familyName":"Takaoka","affiliation":[],"nameIdentifiers":[]},{"name":"Miki, Yoshio","nameType":"Personal","givenName":"Yoshio","familyName":"Miki","affiliation":[],"nameIdentifiers":[]},{"name":"Nakanishi, Akira","nameType":"Personal","givenName":"Akira","familyName":"Nakanishi","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"BRCA2 mediates centrosome cohesion via an interaction with cytoplasmic dynein"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2016-07-19","dateType":"Created"},{"date":"2016-08-15","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2016.1195531","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.3492152","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["246154 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"BRCA2 is responsible for familial breast and ovarian cancer and has been linked to DNA repair and centrosome duplication. Here we analyzed the mechanism by which the centrosomal localization signal (CLS) of BRCA2 interacts with cytoplasmic dynein 1 to localize BRCA2 to the centrosome. \u003ci\u003eIn vitro\u003c/i\u003e pull-down assays demonstrated that BRCA2 directly binds to the cytoplasmic dynein 1 light intermediate chain 2. A dominant-negative HA-CLS-DsRed fusion protein, the depletion of dynein by siRNA, and the inactivation of dynein by EHNA, inhibited the localization of BRCA2 at centrosomes and caused the separation of centrosome pairs during the S-phase. The double depletion of BRCA2 and C-Nap1 caused a larger dispersion of centrosome distances than the silencing of C-Nap1. These results suggest that cytoplasmic dynein 1 binds to BRCA2 through the latter's CLS and BRCA2 mediates the cohesion between centrosomes during the S phase, potentially serving as a cell-cycle checkpoint.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/BRCA2_mediates_centrosome_cohesion_via_an_interaction_with_cytoplasmic_dynein/3492152/1","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":1,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-07-19T22:40:50.000Z","registered":"2016-07-19T22:40:51.000Z","published":"2016","updated":"2020-09-04T05:31:49.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"pr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