{"data":{"id":"10.6084/m9.figshare.3487649","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3487649","prefix":"10.6084","suffix":"m9.figshare.3487649","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Poole, Aaron R.","nameType":"Personal","givenName":"Aaron R.","familyName":"Poole","affiliation":[],"nameIdentifiers":[]},{"name":"Isioma I. Enwerem","affiliation":[],"nameIdentifiers":[]},{"name":"Vicino, Ian A.","nameType":"Personal","givenName":"Ian A.","familyName":"Vicino","affiliation":[],"nameIdentifiers":[]},{"name":"Coole, Jackson B.","nameType":"Personal","givenName":"Jackson B.","familyName":"Coole","affiliation":[],"nameIdentifiers":[]},{"name":"Smith, Stanley V.","nameType":"Personal","givenName":"Stanley V.","familyName":"Smith","affiliation":[],"nameIdentifiers":[]},{"name":"Hebert, Michael D.","nameType":"Personal","givenName":"Michael D.","familyName":"Hebert","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Identification of processing elements and interactors implicate SMN, coilin and the pseudogene-encoded coilp1 in telomerase and box C/D scaRNP biogenesis"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Neuroscience"},{"subject":"Pharmacology"},{"subject":"Cancer"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"}],"contributors":[],"dates":[{"date":"2016-07-15","dateType":"Created"},{"date":"2016-09-30","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2016.1211224","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["614024 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Many cellular functions, such as translation, require ribonucleoproteins (RNPs). The biogenesis of RNPs is a multi-step process that, depending on the RNP, can take place in many cellular compartments. Here we examine 2 different RNPs: telomerase and small Cajal body-specific RNPs (scaRNPs). Both of these RNPs are enriched in the Cajal body (CB), which is a subnuclear domain that also has high concentrations of another RNP, small nuclear RNPs (snRNPs). SnRNPs are essential components of the spliceosome, and scaRNPs modify the snRNA component of the snRNP. The CB contains many proteins, including WRAP53, SMN and coilin, the CB marker protein. We show here that coilin, SMN and coilp1, a newly identified protein encoded by a pseudogene in human, associate with telomerase RNA and a subset of scaRNAs. We also have identified a processing element within box C/D scaRNA. Our findings thus further strengthen the connection between the CB proteins coilin and SMN in the biogenesis of telomeras e and box C/D scaRNPs, and reveal a new player, coilp1, that likely participates in this process.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Identification_of_processing_elements_and_interactors_implicate_SMN_coilin_and_the_pseudogene-encoded_coilp1_in_telomerase_and_box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