{"data":{"id":"10.6084/m9.figshare.3479609","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3479609","prefix":"10.6084","suffix":"m9.figshare.3479609","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Watanabe, Seiya","nameType":"Personal","givenName":"Seiya","familyName":"Watanabe","affiliation":[],"nameIdentifiers":[]},{"name":"Utsumi, Yuki","nameType":"Personal","givenName":"Yuki","familyName":"Utsumi","affiliation":[],"nameIdentifiers":[]},{"name":"Sawayama, Shigeki","nameType":"Personal","givenName":"Shigeki","familyName":"Sawayama","affiliation":[],"nameIdentifiers":[]},{"name":"Watanabe, Yasuo","nameType":"Personal","givenName":"Yasuo","familyName":"Watanabe","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Identification and characterization of d-arabinose reductase and d-arabinose transporters from \u003ci\u003ePichia stipitis\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Genetics"},{"subject":"Biotechnology"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2016-07-11","dateType":"Created"},{"date":"2016-09-26","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2016.1204221","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["46151 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"d-xylose and l-arabinose are the major constituents of plant lignocelluloses, and the related fungal metabolic pathways have been extensively examined. Although \u003ci\u003ePichia stipitis\u003c/i\u003e CBS 6054 grows using d-arabinose as the sole carbon source, the hypothetical pathway has not yet been clarified at the molecular level. We herein purified NAD(P)H-dependent d-arabinose reductase from cells grown on d-arabinose, and found that the enzyme was identical to the known d-xylose reductase (XR). The enzyme activity of XR with d-arabinose was previously reported to be only 1% that with d-xylose. The \u003ci\u003ek\u003c/i\u003e\u003csub\u003ecat\u003c/sub\u003e/\u003ci\u003eK\u003c/i\u003e\u003csub\u003em\u003c/sub\u003e value with d-arabinose (1.27 min\u003csup\u003e−1\u003c/sup\u003e mM\u003csup\u003e−1\u003c/sup\u003e), which was determined using the recombinant enzyme, was 13.6- and 10.5-fold lower than those with l-arabinose and d-xylose, respectively. Among the 34 putative sugar transporters from \u003ci\u003eP. stipitis\u003c/i\u003e, only seven genes exhibited uptake ability not only for d-arabinose, but also for d-glucose and other pentose sugars including d-xylose and l-arabinose in \u003ci\u003eSaccharomyces cerevisiae\u003c/i\u003e. d-Xylose reductase, encoded by \u003ci\u003eXYL1\u003c/i\u003e gene, plays as an aldose reductase involved not only in d-xylose and l-arabinose but also d-arabinose metabolism.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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