{"data":{"id":"10.6084/m9.figshare.3470111","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3470111","prefix":"10.6084","suffix":"m9.figshare.3470111","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Viroj Boonyaratanakornkit","affiliation":[],"nameIdentifiers":[]},{"name":"Sutthirat Udommethaporn","affiliation":[],"nameIdentifiers":[]},{"name":"Tewin Tencomnao","affiliation":[],"nameIdentifiers":[]},{"name":"McGowan, Eileen M.","nameType":"Personal","givenName":"Eileen M.","familyName":"McGowan","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Tet-inducible Keratinocytes as A Novel Tool for Assessment of Anti-TNFα Activities"}],"publisher":"figshare","container":{},"publicationYear":2016,"subjects":[{"subject":"30405 Molecular Medicine","subjectScheme":"FOR"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Basic medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"60111 Signal Transduction","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2016-07-02","dateType":"Created"},{"date":"2016-07-02","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["6934484 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Detail method for construction of a Tet-inducible keratinocyte cell model for assessing TNF-α activities is shown in Methods file. Supporting data demonstrating inducible TNF-α in the membrane fraction and secreted into the medium is shown in Figure 1. Data demonstrating that Tet-inducible TNF-α could induce pro-inflammatory cytokines similar to recombinant TNF-α is shown in Figure 2. Data demonstrating that our keratinocytes expressing Tet-inducible TNF-α cell model could be used to assess anti-TNFα activites is shown in Figure 3.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/dataset/Tet-inducible_Keratinocytes_as_A_Novel_Tool_for_Assessment_of_Anti-TNF_Activities/3470111","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-07-02T11:41:14.000Z","registered":"2016-07-02T11:41:15.000Z","published":"2016","updated":"2020-09-04T07:00:07.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.3470111","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}