{"data":{"id":"10.6084/m9.figshare.3205216.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3205216.v1","prefix":"10.6084","suffix":"m9.figshare.3205216.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Okimura, Chika","nameType":"Personal","givenName":"Chika","familyName":"Okimura","affiliation":[],"nameIdentifiers":[]},{"name":"Iwadate, Yoshiaki","nameType":"Personal","givenName":"Yoshiaki","familyName":"Iwadate","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Biotechnology"},{"subject":"Developmental Biology"},{"subject":"Hematology"}],"contributors":[],"dates":[{"date":"2016-04-28","dateType":"Created"},{"date":"2017-12-14","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/19336918.2016.1170268","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.3205216","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1126084 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Crawling cells can generate polarity for migration in response to forces applied from the substratum. Such reaction varies according to cell type: there are both fast- and slow-crawling cells. In response to periodic stretching of the elastic substratum, the intracellular stress fibers in slow-crawling cells, such as fibroblasts, rearrange themselves perpendicular to the direction of stretching, with the result that the shape of the cells extends in that direction; whereas fast-crawling cells, such as neutrophil-like differentiated HL-60 cells and \u003ci\u003eDictyostelium\u003c/i\u003e cells, which have no stress fibers, migrate perpendicular to the stretching direction. Fish epidermal keratocytes are another type of fast-crawling cell. However, they have stress fibers in the cell body, which gives them a typical slow-crawling cell structure. In response to periodic stretching of the elastic substratum, intact keratocytes rearrange their stress fibers perpendicular to the direction of stretching in the same way as fibroblasts and migrate parallel to the stretching direction, while blebbistatin-treated stress fiber-less keratocytes migrate perpendicular to the stretching direction, in the same way as seen in HL-60 cells and \u003ci\u003eDictyostelium\u003c/i\u003e cells. Our results indicate that keratocytes have a hybrid mechanosensing system that comprises elements of both fast- and slow-crawling cells, to generate the polarity needed for migration.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/Hybrid_mechanosensing_system_to_generate_the_polarity_needed_for_migration_in_fish_keratocytes/3205216/1","contentUrl":null,"metadataVersion":4,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":n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