{"data":{"id":"10.6084/m9.figshare.3179938.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3179938.v1","prefix":"10.6084","suffix":"m9.figshare.3179938.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Rivera-Casas, Ciro","nameType":"Personal","givenName":"Ciro","familyName":"Rivera-Casas","affiliation":[],"nameIdentifiers":[]},{"name":"Gonzalez-Romero, Rodrigo","nameType":"Personal","givenName":"Rodrigo","familyName":"Gonzalez-Romero","affiliation":[],"nameIdentifiers":[]},{"name":"Cheema, Manjinder S.","nameType":"Personal","givenName":"Manjinder S.","familyName":"Cheema","affiliation":[],"nameIdentifiers":[]},{"name":"Ausió, Juan","nameType":"Personal","givenName":"Juan","familyName":"Ausió","affiliation":[],"nameIdentifiers":[]},{"name":"Eirín-López, José M.","nameType":"Personal","givenName":"José M.","familyName":"Eirín-López","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The characterization of macroH2A beyond vertebrates supports an ancestral origin and conserved role for histone variants in chromatin"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Evolutionary Biology"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Sociology"},{"subject":"FOS: Sociology","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Sociology","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-04-15","dateType":"Created"},{"date":"2016-05-12","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15592294.2016.1172161","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.3179938","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["526450 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Histone variants play a critical role in chromatin structure and epigenetic regulation. These “deviant” proteins have been historically considered as the evolutionary descendants of ancestral canonical histones, helping specialize the nucleosome structure during eukaryotic evolution. Such view is now challenged by 2 major observations: first, canonical histones present extremely unique features not shared with any other genes; second, histone variants are widespread across many eukaryotic groups. The present work further supports the ancestral nature of histone variants by providing the first \u003ci\u003ein vivo\u003c/i\u003e characterization of a functional macroH2A histone (a variant long defined as a specific refinement of vertebrate chromatin) in a non-vertebrate organism (the mussel \u003ci\u003eMytilus\u003c/i\u003e) revealing its recruitment into heterochromatic fractions of actively proliferating tissues. Combined with \u003ci\u003ein silico\u003c/i\u003e analyses of genomic data, these results provide evidence for the widespread presence of macroH2A in metazoan animals, as well as in the holozoan \u003ci\u003eCapsaspora\u003c/i\u003e, supporting an evolutionary origin for this histone variant lineage before the radiation of Filozoans (including Filasterea, Choanoflagellata and Metazoa). Overall, the results presented in this work help configure a new evolutionary scenario in which histone variants, rather than modern “deviants” of canonical histones, would constitute ancient components of eukaryotic chromatin.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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