{"data":{"id":"10.6084/m9.figshare.3166843.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3166843.v1","prefix":"10.6084","suffix":"m9.figshare.3166843.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Pant, Shankar R.","nameType":"Personal","givenName":"Shankar R.","familyName":"Pant","affiliation":[],"nameIdentifiers":[]},{"name":"McNeece, Brant T.","nameType":"Personal","givenName":"Brant T.","familyName":"McNeece","affiliation":[],"nameIdentifiers":[]},{"name":"Keshav Sharma","affiliation":[],"nameIdentifiers":[]},{"name":"Niruala, Prakash","nameType":"Personal","givenName":"Prakash","familyName":"Niruala","affiliation":[],"nameIdentifiers":[]},{"name":"Burson, Hannah E.","nameType":"Personal","givenName":"Hannah E.","familyName":"Burson","affiliation":[],"nameIdentifiers":[]},{"name":"Lawrence, Gary W.","nameType":"Personal","givenName":"Gary W.","familyName":"Lawrence","affiliation":[],"nameIdentifiers":[]},{"name":"Klink, Vincent P.","nameType":"Personal","givenName":"Vincent P.","familyName":"Klink","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The heterologous expression of a \u003ci\u003eGlycine max\u003c/i\u003e homolog of NONEXPRESSOR OF PR1 (NPR1) and α-hydroxynitrile glucosidase suppresses parasitism by the root pathogen \u003ci\u003eMeloidogyne incognita\u003c/i\u003e in \u003ci\u003eGossypium hirsutum\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Ecology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-04-08","dateType":"Created"},{"date":"2016-12-17","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/17429145.2016.1163423","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.3166843","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["52056 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Experiments in \u003ci\u003eGlycine max\u003c/i\u003e (soybean) identified the expression of the salicylic acid signaling and defense gene NONEXPRESSOR OF PR1 (NPR1) in root cells (i.e., syncytium) parasitized by the plant parasitic nematode \u003ci\u003eHeterodera glycines\u003c/i\u003e undergoing the process of resistance. Gm-NPR1-2 overexpression in \u003ci\u003eG. max\u003c/i\u003e effectively suppresses parasitism by \u003ci\u003eH. glycines\u003c/i\u003e. The heterologous expression of Gm-NPR1-2 in \u003ci\u003eGossypium hirsutum\u003c/i\u003e impairs the ability of the parasitic nematode \u003ci\u003eMeloidogyne incognita\u003c/i\u003e to form root galls, egg sacs, eggs and second-stage juvenile (J2) nematodes. In related experiments, a \u003ci\u003eG. max\u003c/i\u003e β-glycosidase (Gm-βg-4) related to \u003ci\u003eLotus japonicus\u003c/i\u003e secreted defense gene α-hydroxynitrile glucosidase LjBGD7 suppresses \u003ci\u003eM. incognita\u003c/i\u003e parasitism. The results identify a cumulative negative effect that the transgenes have on \u003ci\u003eM. incognita\u003c/i\u003e parasitism and demonstrate that the \u003ci\u003eG. max\u003c/i\u003e–\u003ci\u003eH. glycines\u003c/i\u003e pathosystem is a useful tool to identify defense genes that function in other agriculturally relevant plant species to plant parasitic nematodes with different strategies of parasitism.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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