{"data":{"id":"10.6084/m9.figshare.3100288","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3100288","prefix":"10.6084","suffix":"m9.figshare.3100288","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Naz, Huma","nameType":"Personal","givenName":"Huma","familyName":"Naz","affiliation":[],"nameIdentifiers":[]},{"name":"Mohd. Shahbaaz","affiliation":[],"nameIdentifiers":[]},{"name":"Md. Anzarul Haque","affiliation":[],"nameIdentifiers":[]},{"name":"Bisetty, Krishna","nameType":"Personal","givenName":"Krishna","familyName":"Bisetty","affiliation":[],"nameIdentifiers":[]},{"name":"Asimul Islam","affiliation":[],"nameIdentifiers":[]},{"name":"Faizan Ahmad","affiliation":[],"nameIdentifiers":[]},{"name":"Md. Imtaiyaz Hassan","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Urea-induced denaturation of human calcium/calmodulin-dependent protein kinase IV: a combined spectroscopic and MD simulation studies"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-03-08","dateType":"Created"},{"date":"2017-02-21","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/07391102.2016.1150203","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["10943445 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Calcium/calmodulin-dependent protein kinase IV (CaMKIV) is a multifunctional enzyme which belongs to the Ser/Thr kinase family. CaMKIV plays important role in varieties of biological processes such as gene expression regulation, memory consolidation, bone growth, T-cell maturation, sperm motility, regulation of microtubule dynamics, cell-cycle progression, and apoptosis. To measure stability parameters, urea-induced denaturation of CaMKIV was carried out at pH 7.4 and 25°C, using three different probes, namely far-UV CD, near-UV absorption, and tryptophan fluorescence. A coincidence of normalized denaturation curves of these optical properties suggests that urea-induced denaturation is a two-state process. Analysis of these denaturation curves gave values of 4.20 ± 0.12 kcal mol\u003csup\u003e−1\u003c/sup\u003e, 2.95 ± 0.15 M, and 1.42 ± 0.06 kcal mol\u003csup\u003e−1\u003c/sup\u003e M\u003csup\u003e−1\u003c/sup\u003e for (Gibbs free energy change (Δ\u003ci\u003eG\u003c/i\u003e\u003csub\u003eD\u003c/sub\u003e) in the absence of urea), \u003ci\u003eC\u003c/i\u003e\u003csub\u003em\u003c/sub\u003e (molar urea concentration ([urea]) at the midpoint of the denaturation curve), and \u003ci\u003em\u003c/i\u003e (=∂Δ\u003ci\u003eG\u003c/i\u003e\u003csub\u003eD\u003c/sub\u003e/∂[urea]), respectively. All these experimental observations have been fully supported by 30 ns molecular dynamics simulation studies.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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