{"data":{"id":"10.6084/m9.figshare.2065623.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.2065623.v1","prefix":"10.6084","suffix":"m9.figshare.2065623.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Lv, Qingyan","givenName":"Qingyan","familyName":"Lv","affiliation":[],"nameIdentifiers":[]},{"name":"Yuan, Lin","givenName":"Lin","familyName":"Yuan","affiliation":[],"nameIdentifiers":[]},{"name":"Song, Yuning","givenName":"Yuning","familyName":"Song","affiliation":[],"nameIdentifiers":[]},{"name":"Sui, Tingting","givenName":"Tingting","familyName":"Sui","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Zhanjun","givenName":"Zhanjun","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Lai, Liangxue","givenName":"Liangxue","familyName":"Lai","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"D-repeat in the \u003ci\u003eXIST\u003c/i\u003e gene is required for X chromosome inactivation"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Genetics"},{"subject":"Molecular Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Science Policy"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-01-20","dateType":"Created"},{"date":"2023-05-30","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.2065623","relatedIdentifierType":"DOI"},{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1137420","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["4386124 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eXIST\u003c/i\u003e is a long non-coding RNA, which expressed exclusively from the inactive X chromosome. Although it has been revealed that the A-repeat contributes to the X chromosome inactivation (X-inactivation), the role of the longest D-repeat has not yet been investigated. Here, a sgRNA directed CRISPR/Cas9 system which have multiple target sites within repeat D of \u003ci\u003eXIST\u003c/i\u003e, were used to generate D-repeat deletion and studied its roles on X-inactivation. The results showed that the deletion of D-repeat caused a significantly decreased expression of \u003ci\u003eXIST\u003c/i\u003e, and up regulated expression of X-linked genes, suggesting that the D-repeat may play an important role in the regulation of \u003ci\u003eXIST\u003c/i\u003e expression and silencing of the X-linked genes, which could provide a new idea in the molecular mechanisms of X-inactivation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/D_repeat_in_the_i_XIST_i_gene_is_required_for_X_chromosome_inactivation/2065623/1","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":1,"citationsOverTime":[{"year":"2024","total":1}],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-20T05:54:21.000Z","registered":"2016-01-20T05:54:22.000Z","published":"2016","updated":"2024-03-24T12:34:01.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.2065623.v1","type":"media"}},"references":{"data":[]},"citations":{"data":[{"id":"10.1080/15476286.2015.1137420","type":"dois"}]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}