{"data":{"id":"10.6084/m9.figshare.2064693.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.2064693.v1","prefix":"10.6084","suffix":"m9.figshare.2064693.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Borodkina, Aleksandra V.","nameType":"Personal","givenName":"Aleksandra V.","familyName":"Borodkina","affiliation":[],"nameIdentifiers":[]},{"name":"Shatrova, Alla N.","nameType":"Personal","givenName":"Alla N.","familyName":"Shatrova","affiliation":[],"nameIdentifiers":[]},{"name":"Deryabin, Pavel I.","nameType":"Personal","givenName":"Pavel I.","familyName":"Deryabin","affiliation":[],"nameIdentifiers":[]},{"name":"Grukova, Anastasiya A.","nameType":"Personal","givenName":"Anastasiya A.","familyName":"Grukova","affiliation":[],"nameIdentifiers":[]},{"name":"Nikolsky, Nikolay N.","nameType":"Personal","givenName":"Nikolay N.","familyName":"Nikolsky","affiliation":[],"nameIdentifiers":[]},{"name":"Burova, Elena B.","nameType":"Personal","givenName":"Elena B.","familyName":"Burova","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Tetraploidization or autophagy: The ultimate fate of senescent human endometrial stem cells under ATM or p53 inhibition"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2016-01-18","dateType":"Created"},{"date":"2019-10-25","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1121326","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.2064693","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["14571802 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"ABSTRACTPreviously we demonstrated that endometrium-derived human mesenchymal stem cells (hMESCs) via activation of the ATM/p53/p21/Rb pathway enter the premature senescence in response to oxidative stress. Down regulation effects of the key components of this signaling pathway, particularly ATM and p53, on a fate of stressed hMESCs have not yet been investigated. In the present study by using the specific inhibitors Ku55933 and Pifithrin-α, we confirmed implication of both ATM and p53 in H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-induced senescence of hMESCs. ATM or p53 down regulation was shown to modulate differently the cellular fate of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-treated hMESCs. ATM inhibition allowed H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-stimulated hMESCs to escape the permanent cell cycle arrest due to loss of the functional ATM/p53/p21/Rb pathway, and induced bypass of mitosis and re-entry into S phase, resulting in tetraploid cells. On the contrary, suppression of the p53 transcriptional activity caused a pronounced cell death of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-treated hMESCs via autophagy induction. The obtained data clearly demonstrate that down regulation of ATM or p53 shifts senescence of human endometrial stem cells toward tetraploidization or autophagy.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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