{"data":{"id":"10.6084/m9.figshare.2064693","type":"dois","attributes":{"doi":"10.6084/m9.figshare.2064693","prefix":"10.6084","suffix":"m9.figshare.2064693","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Borodkina, Aleksandra V.","nameType":"Personal","givenName":"Aleksandra V.","familyName":"Borodkina","affiliation":[],"nameIdentifiers":[]},{"name":"Shatrova, Alla N.","nameType":"Personal","givenName":"Alla N.","familyName":"Shatrova","affiliation":[],"nameIdentifiers":[]},{"name":"Deryabin, Pavel I.","nameType":"Personal","givenName":"Pavel I.","familyName":"Deryabin","affiliation":[],"nameIdentifiers":[]},{"name":"Grukova, Anastasiya A.","nameType":"Personal","givenName":"Anastasiya A.","familyName":"Grukova","affiliation":[],"nameIdentifiers":[]},{"name":"Nikolsky, Nikolay N.","nameType":"Personal","givenName":"Nikolay N.","familyName":"Nikolsky","affiliation":[],"nameIdentifiers":[]},{"name":"Burova, Elena B.","nameType":"Personal","givenName":"Elena B.","familyName":"Burova","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Tetraploidization or autophagy: The ultimate fate of senescent human endometrial stem cells under ATM or p53 inhibition"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2019,"subjects":[{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-01-18","dateType":"Created"},{"date":"2019-10-25","dateType":"Updated"},{"date":"2019","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1121326","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["7291938 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Previously we demonstrated that endometrium-derived human mesenchymal stem cells (hMESCs) via activation of the ATM/p53/p21/Rb pathway enter the premature senescence in response to oxidative stress. Down regulation effects of the key components of this signaling pathway, particularly ATM and p53, on a fate of stressed hMESCs have not yet been investigated. In the present study by using the specific inhibitors Ku55933 and Pifithrin-α, we confirmed implication of both ATM and p53 in H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-induced senescence of hMESCs. ATM or p53 down regulation was shown to modulate differently the cellular fate of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-treated hMESCs. ATM inhibition allowed H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-stimulated hMESCs to escape the permanent cell cycle arrest due to loss of the functional ATM/p53/p21/Rb pathway, and induced bypass of mitosis and re-entry into S phase, resulting in tetraploid cells. On the contrary, suppression of the p53 transcriptional activity caused a pronounced cell death of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-treated hMESCs via autophagy induction. The obtained data clearly demonstrate that down regulation of ATM or p53 shifts senescence of human endometrial stem cells toward tetraploidization or autophagy.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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