{"data":{"id":"10.6084/m9.figshare.2064498.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.2064498.v1","prefix":"10.6084","suffix":"m9.figshare.2064498.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Ceyla Yorucu","affiliation":[],"nameIdentifiers":[]},{"name":"Lau, Katherine","nameType":"Personal","givenName":"Katherine","familyName":"Lau","affiliation":[],"nameIdentifiers":[]},{"name":"Mittar, Shweta","nameType":"Personal","givenName":"Shweta","familyName":"Mittar","affiliation":[],"nameIdentifiers":[]},{"name":"Green, Nicola H.","nameType":"Personal","givenName":"Nicola H.","familyName":"Green","affiliation":[],"nameIdentifiers":[]},{"name":"Ahtasham Raza","affiliation":[],"nameIdentifiers":[]},{"name":"Ihtesham Ur Rehman","affiliation":[],"nameIdentifiers":[]},{"name":"MacNeil, Sheila","nameType":"Personal","givenName":"Sheila","familyName":"MacNeil","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue-engineered melanoma models"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"Space Science"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Pharmacology"},{"subject":"Biotechnology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2016-01-18","dateType":"Created"},{"date":"2016-03-21","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/05704928.2015.1126840","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.2064498","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["834277 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Invasion of melanoma cells from the primary tumor involves interaction with adjacent tissues and extracellular matrix. The extent of this interaction is not fully understood. In this study Raman spectroscopy was applied to cryo-sections of established 3D models of melanoma in human skin. Principal component analysis was used to investigate differences between the tumor and normal tissue and between the peri-tumor area and the normal skin. Two human melanoma cells lines A375SM and C8161 were investigated and compared in 3D melanoma models. Changes were found in protein conformations and tryptophan configurations across the entire melanoma samples, in tyrosine orientation and in more fluid lipid packing only in tumor dense areas, and in increased glycogen content in the peri-tumor areas of melanoma. Raman spectroscopy revealed changes around the perimeter of a melanoma tumor as well as detecting differences between the tumor and the normal tissue.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/Raman_spectroscopy_detects_melanoma_and_the_tissue_surr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