{"data":{"id":"10.6084/m9.figshare.1632784","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1632784","prefix":"10.6084","suffix":"m9.figshare.1632784","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Goh, Grace H.","nameType":"Personal","givenName":"Grace H.","familyName":"Goh","affiliation":[],"nameIdentifiers":[]},{"name":"Mark, Peter J.","nameType":"Personal","givenName":"Peter J.","familyName":"Mark","affiliation":[],"nameIdentifiers":[]},{"name":"Maloney, Shane K.","nameType":"Personal","givenName":"Shane K.","familyName":"Maloney","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Altered energy intake and the amplitude of the body temperature rhythm are associated with changes in phase, but not amplitude, of clock gene expression in the rat suprachiasmatic nucleus \u003ci\u003ein vivo\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Neuroscience"},{"subject":"Physiology"},{"subject":"Evolutionary Biology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Cancer"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2016-01-08","dateType":"Created"},{"date":"2016-02-18","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/07420528.2015.1112395","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1105864 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Circadian rhythms in mammals are driven by a central clock in the suprachiasmatic nucleus (SCN). \u003ci\u003eIn vitro\u003c/i\u003e, temperature cycles within the physiological range can act as potent entraining cues for biological clocks. We altered the body temperature (T\u003csub\u003ec\u003c/sub\u003e) rhythm in rats by manipulating energy intake (EI) to determine whether EI-induced changes in T\u003csub\u003ec\u003c/sub\u003e oscillations are associated with changes in SCN clock gene rhythms \u003ci\u003ein vivo\u003c/i\u003e. Male Wistar rats (\u003ci\u003en\u003c/i\u003e = 16 per diet) were maintained on either an \u003ci\u003ead libitum\u003c/i\u003e diet (CON), a high energy cafeteria diet (CAF), or a calorie restricted diet (CR), and T\u003csub\u003ec\u003c/sub\u003e was recorded every 30 min for 6-7 weeks. SCN tissue was harvested from rats at \u003ci\u003ezeitgeber\u003c/i\u003e time (ZT) 0, ZT6, ZT12, or ZT18. Expression of the clock genes \u003ci\u003eBmal1, Per2, Cry1\u003c/i\u003e, and \u003ci\u003eRev-erbα\u003c/i\u003e, the heat shock transcription factor \u003ci\u003eHsf1\u003c/i\u003e, and the heat shock protein \u003ci\u003eHsp90aa1\u003c/i\u003e, were determined using qPCR. The circadian profile of gene expression for each gene was characterized using cosinor analysis. Compared to the CON rats, the amplitude of T\u003csub\u003ec\u003c/sub\u003e was decreased in CAF rats by 0.1 °C (\u003ci\u003ep\u003c/i\u003e p Hsp90aa1 expression was lowest in CAF rats and highest in CR rats (\u003ci\u003ep\u003c/i\u003e = 0.045), but the amplitude of all of the clock genes and \u003ci\u003eHsf1\u003c/i\u003e were unaffected by diet (\u003ci\u003ep\u003c/i\u003e \u0026gt; 0.25). Compared to CON, phase advances of the T\u003csub\u003ec\u003c/sub\u003e, \u003ci\u003eBmal1\u003c/i\u003e, and \u003ci\u003ePer2\u003c/i\u003e rhythms were observed with CR feeding (\u003ci\u003ep\u003c/i\u003e in vivo, the SCN is largely resistant to entrainment by EI-induced changes in the T\u003csub\u003ec\u003c/sub\u003e rhythm, although some phase entrainment may occur.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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