{"data":{"id":"10.6084/m9.figshare.1632726","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1632726","prefix":"10.6084","suffix":"m9.figshare.1632726","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Nishimura, Kohji","nameType":"Personal","givenName":"Kohji","familyName":"Nishimura","affiliation":[],"nameIdentifiers":[]},{"name":"Matsunami, Erika","nameType":"Personal","givenName":"Erika","familyName":"Matsunami","affiliation":[],"nameIdentifiers":[]},{"name":"Yoshida, Shohei","nameType":"Personal","givenName":"Shohei","familyName":"Yoshida","affiliation":[],"nameIdentifiers":[]},{"name":"Kohata, Shuhei","nameType":"Personal","givenName":"Shuhei","familyName":"Kohata","affiliation":[],"nameIdentifiers":[]},{"name":"Yamauchi, Junji","nameType":"Personal","givenName":"Junji","familyName":"Yamauchi","affiliation":[],"nameIdentifiers":[]},{"name":"Jisaka, Mitsuo","nameType":"Personal","givenName":"Mitsuo","familyName":"Jisaka","affiliation":[],"nameIdentifiers":[]},{"name":"Nagaya, Tsutomu","nameType":"Personal","givenName":"Tsutomu","familyName":"Nagaya","affiliation":[],"nameIdentifiers":[]},{"name":"Yokota, Kazushige","nameType":"Personal","givenName":"Kazushige","familyName":"Yokota","affiliation":[],"nameIdentifiers":[]},{"name":"Nakagawa, Tsuyoshi","nameType":"Personal","givenName":"Tsuyoshi","familyName":"Nakagawa","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The tyrosine-sorting motif of the vacuolar sorting receptor VSR4 from \u003ci\u003eArabidopsis thaliana\u003c/i\u003e, which is involved in the interaction between VSR4 and AP1M2, μ1-adaptin type 2 of clathrin adaptor complex 1 subunits, participates in the post-Golgi sorting of VSR4"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-01-06","dateType":"Created"},{"date":"2016-04-07","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2015.1116925","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["2767817 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"μ1-Adaptin of adaptor protein (AP) 1 complex, AP1M, is generally accepted to load cargo proteins into clathrin-coated vesicles (CCVs) at the \u003ci\u003etrans\u003c/i\u003e-Golgi network through its binding to cargo-recognition sequences (CRSs). Plant vacuolar-sorting receptors (VSRs) function in sorting vacuolar proteins, which are reportedly mediated by CCV. We herein investigated the involvement of CRSs of \u003ci\u003eArabidopsis thaliana\u003c/i\u003e VSR4 in the sorting of VSR4. The results obtained showed the increased localization of VSR4 at the plasma membrane or vacuoles by mutations in CRSs including the tyrosine-sorting motif YMPL or acidic dileucine-like motif EIRAIM, respectively. Interaction analysis using the bimolecular fluorescence complementation (BiFC) system, V10-BiFC, which we developed, indicated an interaction between VSR4 and AP1M2, AP1M type 2, which was attenuated by a YMPL mutation, but not influenced by an EIRAIM mutation. These results demonstrated the significance of the recognition of YMPL in VSR4 by AP1M2 for the post-Golgi sorting of VSR4. Clathrin AP1M2-recognizable tyrosine motif for plant VSR4 sorting.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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