{"data":{"id":"10.6084/m9.figshare.1632160","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1632160","prefix":"10.6084","suffix":"m9.figshare.1632160","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jiang, Chang","givenName":"Chang","familyName":"Jiang","affiliation":[],"nameIdentifiers":[]},{"name":"Veon, William","givenName":"William","familyName":"Veon","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Hui","givenName":"Hui","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"R Hallows, Kenneth","givenName":"Kenneth","familyName":"R Hallows","affiliation":[],"nameIdentifiers":[]},{"name":"Roy, Partha","givenName":"Partha","familyName":"Roy","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Epithelial morphological reversion drives Profilin-1-induced elevation of p27\u003csup\u003ekip1\u003c/sup\u003e in mesenchymal triple-negative human breast cancer cells through AMP-activated protein kinase activation"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Pharmacology"},{"subject":"Chemical Sciences not elsewhere classified"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"Hematology"},{"subject":"Plant Biology"}],"contributors":[],"dates":[{"date":"2018-08-31","dateType":"Created"},{"date":"2023-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1069929","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["972973 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Profilin-1 (Pfn1) is an important regulator of actin polymerization that is downregulated in human breast cancer. Previous studies have shown Pfn1 has a tumor-suppressive effect on mesenchymal-like triple-negative breast cancer cells, and Pfn1-induced growth suppression is partly mediated by upregulation of cell-cycle inhibitor p27\u003csup\u003ekip1\u003c/sup\u003e (p27). In this study, we demonstrate that Pfn1 overexpression leads to accumulation of p27 through promoting AMPK activation and AMPK-dependent phosphorylation of p27 on T198 residue, a post-translational modification that leads to increased protein stabilization of p27. This pathway is mediated by Pfn1-induced epithelial morphological reversion of mesenchymal breast cancer through cadherin-mediated restoration of adherens junctions. These findings not only elucidate a potential mechanism of how Pfn1 may inhibit proliferation of mesenchymal breast cancer cells, but also highlight a novel pathway of cadherin-mediated p27 induction and therefore cell-cycle control in cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Epithelial_morphological_reversion_drives_Profilin_1_induced_elevation_of_p27_sup_kip1_sup_in_mesenchymal_triple_negative_human_breast_cancer_cells_through_AMP_activated_protein_kinase_activation/1632160","contentUrl":null,"metadataVersion":5,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-05T00:48:50.000Z","registered":"2016-01-05T00:48:51.000Z","published":"2015","updated":"2024-02-07T10:53:32.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1632160","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}