{"data":{"id":"10.6084/m9.figshare.1632152","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1632152","prefix":"10.6084","suffix":"m9.figshare.1632152","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Koch, Henrik","nameType":"Personal","givenName":"Henrik","familyName":"Koch","affiliation":[],"nameIdentifiers":[]},{"name":"Raabe, Monika","nameType":"Personal","givenName":"Monika","familyName":"Raabe","affiliation":[],"nameIdentifiers":[]},{"name":"Urlaub, Henning","nameType":"Personal","givenName":"Henning","familyName":"Urlaub","affiliation":[],"nameIdentifiers":[]},{"name":"Bindereif, Albrecht","nameType":"Personal","givenName":"Albrecht","familyName":"Bindereif","affiliation":[],"nameIdentifiers":[]},{"name":"Preußer, Christian","nameType":"Personal","givenName":"Christian","familyName":"Preußer","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The polyadenylation complex of \u003ci\u003eTrypanosoma brucei:\u003c/i\u003e Characterization of the functional poly(A) polymerase"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"Molecular Biology"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2016-01-05","dateType":"Created"},{"date":"2016-03-17","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1130208","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3477999 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The generation of mature mRNA in the protozoan parasite \u003ci\u003eTrypanosoma brucei\u003c/i\u003e requires coupled polyadenylation and \u003ci\u003etrans\u003c/i\u003e splicing. In contrast to other eukaryotes, we still know very little on components, mechanisms, and dynamics of the 3′ end-processing machinery in trypanosomes. To characterize the catalytic core of the polyadenylation complex in \u003ci\u003eT. brucei\u003c/i\u003e, we first identified the poly(A) polymerase [Tb927.7.3780] as the major functional, nuclear-localized enzyme in trypanosomes. In contrast, another poly(A) polymerase, encoded by an intron-containing gene [Tb927.3.3160], localizes mainly in the cytoplasm and appears not to be functional in general 3′ end processing of mRNAs. Based on tandem-affinity purification with tagged CPSF160 and mass spectrometry, we identified ten associated components of the trypanosome polyadenylation complex, including homologues to all four CPSF subunits, Fip1, CstF50/64, and Symplekin, as well as two hypothetical proteins. RNAi-mediated knockdown revealed that most of these factors are essential for growth and required for both \u003ci\u003ein vivo\u003c/i\u003e polyadenylation and \u003ci\u003etrans\u003c/i\u003e splicing, arguing for a general coupling of these two mRNA-processing 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