{"data":{"id":"10.6084/m9.figshare.1632150","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1632150","prefix":"10.6084","suffix":"m9.figshare.1632150","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Tognon, Emiliana","nameType":"Personal","givenName":"Emiliana","familyName":"Tognon","affiliation":[],"nameIdentifiers":[]},{"name":"Kobia, Francis","nameType":"Personal","givenName":"Francis","familyName":"Kobia","affiliation":[],"nameIdentifiers":[]},{"name":"Busi, Ilaria","nameType":"Personal","givenName":"Ilaria","familyName":"Busi","affiliation":[],"nameIdentifiers":[]},{"name":"Fumagalli, Arianna","nameType":"Personal","givenName":"Arianna","familyName":"Fumagalli","affiliation":[],"nameIdentifiers":[]},{"name":"Masi, Federico De","nameType":"Personal","givenName":"Federico De","familyName":"Masi","affiliation":[],"nameIdentifiers":[]},{"name":"Vaccari, Thomas","nameType":"Personal","givenName":"Thomas","familyName":"Vaccari","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in \u003ci\u003eDrosophila melanogaster\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-01-05","dateType":"Created"},{"date":"2016-04-06","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15548627.2015.1134080","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1589613 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"In vertebrates, TFEB (transcription factor EB) and MITF (microphthalmia-associated transcription factor) family of basic Helix-Loop-Helix (bHLH) transcription factors regulates both lysosomal function and organ development. However, it is not clear whether these 2 processes are interconnected. Here, we show that Mitf, the single TFEB and MITF ortholog in \u003ci\u003eDrosophila\u003c/i\u003e, controls expression of vacuolar-type H\u003csup\u003e+\u003c/sup\u003e-ATPase pump (V-ATPase) subunits. Remarkably, we also find that expression of \u003ci\u003eVha16-1\u003c/i\u003e and \u003ci\u003eVha13\u003c/i\u003e, encoding 2 key components of V-ATPase, is patterned in the wing imaginal disc. In particular, \u003ci\u003eVha16-1\u003c/i\u003e expression follows differentiation of proneural regions of the disc. These regions, which will form sensory organs in the adult, appear to possess a distinctive endolysosomal compartment and Notch (N) localization. Modulation of Mitf activity in the disc in vivo alters endolysosomal function and disrupts proneural patterning. Similar to our findings in \u003ci\u003eDrosophila\u003c/i\u003e, in human breast epithelial cells we observe that impairment of the \u003ci\u003eVha16-1\u003c/i\u003e human ortholog \u003ci\u003eATP6V0C\u003c/i\u003e changes the size and function of the endolysosomal compartment and that depletion of TFEB reduces ligand-independent N signaling activity. Our data suggest that lysosomal-associated functions regulated by the TFEB-V-ATPase axis might play a conserved role in shaping cell fate.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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