{"data":{"id":"10.6084/m9.figshare.1627973","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1627973","prefix":"10.6084","suffix":"m9.figshare.1627973","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Sela T Po‘Uha","affiliation":[],"nameIdentifiers":[]},{"name":"Kavallaris, Maria","nameType":"Personal","givenName":"Maria","familyName":"Kavallaris","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Gamma-actin is involved in regulating centrosome function and mitotic progression in cancer cells"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2015-12-24","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1120920","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3717439 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Reorganization of the actin cytoskeleton during mitosis is crucial for regulating cell division. A functional role for γ-actin in mitotic arrest induced by the microtubule-targeted agent, paclitaxel, has recently been demonstrated. We hypothesized that γ-actin plays a role in mitosis. Herein, we investigated the effect of γ-actin in mitosis and demonstrated that γ-actin is important in the distribution of β-actin and formation of actin-rich retraction fibers during mitosis. The reduced ability of paclitaxel to induce mitotic arrest as a result of γ-actin depletion was replicated with a range of mitotic inhibitors, suggesting that γ-actin loss reduces the ability of broad classes of anti-mitotic agents to induce mitotic arrest. In addition, partial depletion of γ-actin enhanced centrosome amplification in cancer cells and caused a significant delay in prometaphase/metaphase. This prolonged prometaphase/metaphase arrest was due to mitotic defects such as uncongressed and missegregated chromosomes, and correlated with an increased presence of mitotic spindle abnormalities in the γ-actin depleted cells. Collectively, these results demonstrate a previously unknown role for γ-actin in regulating centrosome function, chromosome alignment and maintenance of mitotic spindle integrity.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Gamma_actin_is_involved_in_regulating_centrosome_function_and_mitotic_progression_in_cancer_cells/1627973/1","contentUrl":null,"metadataVersion":8,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":6,"viewsOverTime":[{"yearMonth":"2025-04","total":2},{"yearMonth":"2025-04","total":2},{"yearMonth":"2025-05","total":1},{"yearMonth":"2025-05","total":1}],"downloadCount":2,"downloadsOverTime":[{"yearMonth":"2025-04","total":0},{"yearMonth":"2025-04","total":0},{"yearMonth":"2025-05","total":1},{"yearMonth":"2025-05","total":1}],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-12-24T04:48:53.000Z","registered":"2015-12-24T04:48:54.000Z","published":"2015","updated":"2025-06-01T07:36:14.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1627973","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}