{"data":{"id":"10.6084/m9.figshare.1627969","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1627969","prefix":"10.6084","suffix":"m9.figshare.1627969","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Perucca, Paola","nameType":"Personal","givenName":"Paola","familyName":"Perucca","affiliation":[],"nameIdentifiers":[]},{"name":"Sommatis, Sabrina","nameType":"Personal","givenName":"Sabrina","familyName":"Sommatis","affiliation":[],"nameIdentifiers":[]},{"name":"Mocchi, Roberto","nameType":"Personal","givenName":"Roberto","familyName":"Mocchi","affiliation":[],"nameIdentifiers":[]},{"name":"Prosperi, Ennio","nameType":"Personal","givenName":"Ennio","familyName":"Prosperi","affiliation":[],"nameIdentifiers":[]},{"name":"Stivala, Lucia Anna","nameType":"Personal","givenName":"Lucia Anna","familyName":"Stivala","affiliation":[],"nameIdentifiers":[]},{"name":"Cazzalini, Ornella","nameType":"Personal","givenName":"Ornella","familyName":"Cazzalini","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"A DDB2 mutant protein unable to interact with PCNA promotes cell cycle progression of human transformed embryonic kidney cells"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"Hematology"}],"contributors":[],"dates":[{"date":"2015-12-24","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1120921","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["993957 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"DNA damage binding protein 2 (DDB2) is a protein involved in the early step of DNA damage recognition of the nucleotide excision repair (NER) process. Recently, it has been suggested that DDB2 may play a role in DNA replication, based on its ability to promote cell proliferation. We have previously shown that DDB2 binds PCNA during NER, but also in the absence of DNA damage; however, whether and how this interaction influences cell proliferation is not known. In this study, we have addressed this question by using HEK293 cell clones stably expressing DDB2\u003csup\u003eWt\u003c/sup\u003e protein, or a mutant form (DDB2\u003csup\u003eMut\u003c/sup\u003e) unable to interact with PCNA. We report that overexpression of the DDB2\u003csup\u003eMut\u003c/sup\u003e protein provides a proliferative advantage over the wild type form, by influencing cell cycle progression. In particular, an increase in the number of S-phase cells, together with a reduction in p21\u003csup\u003eCDKN1A\u003c/sup\u003e protein level, and a shorter cell cycle length, has been observed in the DDB2\u003csup\u003eMut\u003c/sup\u003e cells. These results suggest that DDB2 influences cell cycle progression thanks to its interaction with PCNA.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/A_DDB2_mutant_protein_unable_to_interact_with_PCN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