{"data":{"id":"10.6084/m9.figshare.1627968","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1627968","prefix":"10.6084","suffix":"m9.figshare.1627968","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yu, Fujun","givenName":"Fujun","familyName":"Yu","affiliation":[],"nameIdentifiers":[]},{"name":"Lu, Zhongqiu","givenName":"Zhongqiu","familyName":"Lu","affiliation":[],"nameIdentifiers":[]},{"name":"Cai, Jing","givenName":"Jing","familyName":"Cai","affiliation":[],"nameIdentifiers":[]},{"name":"Huang, Kate","givenName":"Kate","familyName":"Huang","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Bicheng","givenName":"Bicheng","familyName":"Chen","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Guojun","givenName":"Guojun","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Dong, Peihong","givenName":"Peihong","familyName":"Dong","affiliation":[],"nameIdentifiers":[]},{"name":"Zheng, Jianjian","givenName":"Jianjian","familyName":"Zheng","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"MALAT1 functions as a competing endogenous RNA to mediate Rac1 expression by sequestering miR-101b in liver fibrosis"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Chemical Sciences not elsewhere classified"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Developmental Biology"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2016-01-05","dateType":"Created"},{"date":"2023-06-03","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1120917","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["14283 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Emerging evidence shows that Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) plays a pivotal role in cell proliferation, migration, and invasion in tumors. However, the biological role and underlying mechanism of MALAT1 in liver fibrosis remains undefined. In this study, up-regulation of MALAT1 was observed in fibrotic liver tissues and in activated hepatic stellate cells (HSCs). In addition, depletion of MALAT1 inhibited the activation of HSCs \u003ci\u003ein vitro\u003c/i\u003e and attenuated collagen deposits \u003ci\u003ein vivo\u003c/i\u003e. Our results demonstrated that MALAT1 expression is negatively correlated with microRNA-101b (miR-101b) expression. Furthermore, there was a negative feedback loop between the levels of MALAT1 and miR-101b. Luciferase reporter assay indicated that MALAT1 and RAS-related C3 botulinum substrate 1 (Rac1) are targets of miR-101b. We uncovered that MALAT1 regulates Rac1 expression through miR-101b as a competing endogenous RNA (ceRNA), thereby influencing the proliferation, cell cycle and activation of primary HSCs. Collectively, The ceRNA regulatory network may prompt a better understanding of liver fibrogenesis and contribute to a novel therapeutic strategy for liver fibrosis.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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