{"data":{"id":"10.6084/m9.figshare.1626655","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1626655","prefix":"10.6084","suffix":"m9.figshare.1626655","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Price, Jordan V.","nameType":"Personal","givenName":"Jordan V.","familyName":"Price","affiliation":[],"nameIdentifiers":[]},{"name":"Dekker, Cornelia L.","nameType":"Personal","givenName":"Cornelia L.","familyName":"Dekker","affiliation":[],"nameIdentifiers":[]},{"name":"Baechler, Emily C.","nameType":"Personal","givenName":"Emily C.","familyName":"Baechler","affiliation":[],"nameIdentifiers":[]},{"name":"Chih Long Liu","affiliation":[],"nameIdentifiers":[]},{"name":"Utz, Paul J.","nameType":"Personal","givenName":"Paul J.","familyName":"Utz","affiliation":[],"nameIdentifiers":[]},{"name":"Haddon, David James","nameType":"Personal","givenName":"David James","familyName":"Haddon","affiliation":[],"nameIdentifiers":[]},{"name":"Jarrell, Justin Ansel","nameType":"Personal","givenName":"Justin Ansel","familyName":"Jarrell","affiliation":[],"nameIdentifiers":[]},{"name":"Diep, Vivian K.","nameType":"Personal","givenName":"Vivian K.","familyName":"Diep","affiliation":[],"nameIdentifiers":[]},{"name":"Wand, Hannah E.","nameType":"Personal","givenName":"Hannah E.","familyName":"Wand","affiliation":[],"nameIdentifiers":[]},{"name":"Tangsombatvisit, Stephanie","nameType":"Personal","givenName":"Stephanie","familyName":"Tangsombatvisit","affiliation":[],"nameIdentifiers":[]},{"name":"Credo, Grace M.","nameType":"Personal","givenName":"Grace M.","familyName":"Credo","affiliation":[],"nameIdentifiers":[]},{"name":"Mackey, Sally","nameType":"Personal","givenName":"Sally","familyName":"Mackey","affiliation":[],"nameIdentifiers":[]},{"name":"Madoo Varma","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Mapping epitopes of U1-70K autoantibodies at single-amino acid resolution"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"Chemistry"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Medicine"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-12-19","dateType":"Created"},{"date":"2015-12-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/08916934.2015.1077233","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["9631295 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The mechanisms underlying development of ribonucleoprotein (RNP) autoantibodies are unclear. The U1-70K protein is the predominant target of RNP autoantibodies, and the RNA binding domain has been shown to be the immunodominant autoantigenic region of U1-70K, although the specific epitopes are not known. To precisely map U1-70K epitopes, we developed silicon-based peptide microarrays with \u0026gt;5700 features, corresponding to 843 unique peptides derived from the U1-70K protein. The microarrays feature overlapping peptides, with single-amino acid resolution in length and location, spanning amino acids 110–170 within the U1-70K RNA binding domain. We evaluated the serum IgG of a cohort of patients with systemic lupus erythematosus (SLE; \u003ci\u003en\u003c/i\u003e = 26) using the microarrays, and identified multiple reactive epitopes, including peptides 116–121 and 143–148. Indirect peptide ELISA analysis of the sera of patients with SLE (\u003ci\u003en\u003c/i\u003e = 88) revealed that ∼14% of patients had serum IgG reactivity to 116–121, while reactivity to 143–148 appeared to be limited to a single patient. SLE patients with serum reactivity to 116–121 had significantly lower SLE Disease Activity Index (SLEDAI) scores at the time of sampling, compared to non-reactive patients. Minimal reactivity to the peptides was observed in the sera of healthy controls (\u003ci\u003en\u003c/i\u003e = 92). Competitive ELISA showed antibodies to 116–121 bind a common epitope in U1-70K (68–72) and the matrix protein M1 of human influenza B viruses. Institutional Review Boards approved this study. Knowledge of the precise epitopes of U1-70K autoantibodies may provide insight into the mechanisms of development of anti-RNP, identify potential clinical biomarkers and inform ongoing clinical trails of peptide-based therapeutics.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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