{"data":{"id":"10.6084/m9.figshare.1625303","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1625303","prefix":"10.6084","suffix":"m9.figshare.1625303","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Catalán, Julia","nameType":"Personal","givenName":"Julia","familyName":"Catalán","affiliation":[],"nameIdentifiers":[]},{"name":"Siivola, Kirsi M.","nameType":"Personal","givenName":"Kirsi M.","familyName":"Siivola","affiliation":[],"nameIdentifiers":[]},{"name":"Nymark, Penny","nameType":"Personal","givenName":"Penny","familyName":"Nymark","affiliation":[],"nameIdentifiers":[]},{"name":"Lindberg, Hanna","nameType":"Personal","givenName":"Hanna","familyName":"Lindberg","affiliation":[],"nameIdentifiers":[]},{"name":"Suhonen, Satu","nameType":"Personal","givenName":"Satu","familyName":"Suhonen","affiliation":[],"nameIdentifiers":[]},{"name":"Järventaus, Hilkka","nameType":"Personal","givenName":"Hilkka","familyName":"Järventaus","affiliation":[],"nameIdentifiers":[]},{"name":"Koivisto, Antti J.","nameType":"Personal","givenName":"Antti J.","familyName":"Koivisto","affiliation":[],"nameIdentifiers":[]},{"name":"Moreno, Carlos","nameType":"Personal","givenName":"Carlos","familyName":"Moreno","affiliation":[],"nameIdentifiers":[]},{"name":"Vanhala, Esa","nameType":"Personal","givenName":"Esa","familyName":"Vanhala","affiliation":[],"nameIdentifiers":[]},{"name":"Wolff, Henrik","nameType":"Personal","givenName":"Henrik","familyName":"Wolff","affiliation":[],"nameIdentifiers":[]},{"name":"Kling, Kirsten I.","nameType":"Personal","givenName":"Kirsten I.","familyName":"Kling","affiliation":[],"nameIdentifiers":[]},{"name":"Jensen, Keld Alstrup","nameType":"Personal","givenName":"Keld Alstrup","familyName":"Jensen","affiliation":[],"nameIdentifiers":[]},{"name":"Savolainen, Kai","nameType":"Personal","givenName":"Kai","familyName":"Savolainen","affiliation":[],"nameIdentifiers":[]},{"name":"Norppa, Hannu","nameType":"Personal","givenName":"Hannu","familyName":"Norppa","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"\u003ci\u003eIn vitro\u003c/i\u003e and \u003ci\u003ein vivo\u003c/i\u003e genotoxic effects of straight versus tangled multi-walled carbon nanotubes"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"Space Science"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"Pharmacology"},{"subject":"Biotechnology"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"}],"contributors":[],"dates":[{"date":"2015-12-16","dateType":"Created"},{"date":"2016-05-11","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/17435390.2015.1132345","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["12584824 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Some multi-walled carbon nanotubes (MWCNTs) induce mesothelioma in rodents, straight MWCNTs showing a more pronounced effect than tangled MWCNTs. As primary and secondary genotoxicity may play a role in MWCNT carcinogenesis, we used a battery of assays for DNA damage and micronuclei to compare the genotoxicity of straight (MWCNT-S) and tangled MWCNTs (MWCNT-T) \u003ci\u003ein vitro\u003c/i\u003e (primary genotoxicity) and \u003ci\u003ein vivo\u003c/i\u003e (primary or secondary genotoxicity). C57Bl/6 mice showed a dose-dependent increase in DNA strand breaks, as measured by the comet assay, in lung cells 24 h after a single pharyngeal aspiration of MWCNT-S (1–200 μg/mouse). An increase was also observed for DNA strand breaks in lung and bronchoalveolar lavage (BAL) cells and for micronucleated alveolar type II cells in mice exposed to aerosolized MWCNT-S (8.2–10.8 mg/m\u003csup\u003e3\u003c/sup\u003e) for 4 d, 4 h/d. No systemic genotoxic effects, assessed by the γ-H2AX assay in blood mononuclear leukocytes or by micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow or blood, were observed for MWCNT-S by either exposure technique. MWCNT-T showed a dose-related \u003ci\u003edecrease\u003c/i\u003e in DNA damage in BAL and lung cells of mice after a single pharyngeal aspiration (1–200 μg/mouse) and in MNPCEs after inhalation exposure (17.5 mg/m\u003csup\u003e3\u003c/sup\u003e). \u003ci\u003eIn vitro\u003c/i\u003e in human bronchial epithelial BEAS-2B cells, MWCNT-S induced DNA strand breaks at low doses (5 and 10 μg/cm\u003csup\u003e2\u003c/sup\u003e), while MWCNT-T increased strand breakage only at 200 μg/cm\u003csup\u003e2\u003c/sup\u003e. Neither of the MWCNTs was able to induce micronuclei \u003ci\u003ein vitro\u003c/i\u003e. Our findings suggest that both primary and secondary mechanisms may be involved in the genotoxicity of straight MWCNTs.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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