{"data":{"id":"10.6084/m9.figshare.1623156","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1623156","prefix":"10.6084","suffix":"m9.figshare.1623156","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"MacDonald, Chris","nameType":"Personal","givenName":"Chris","familyName":"MacDonald","affiliation":[],"nameIdentifiers":[]},{"name":"Stamnes, Mark A","nameType":"Personal","givenName":"Mark A","familyName":"Stamnes","affiliation":[],"nameIdentifiers":[]},{"name":"Katzmann, David J","nameType":"Personal","givenName":"David J","familyName":"Katzmann","affiliation":[],"nameIdentifiers":[]},{"name":"Piper, Robert C","nameType":"Personal","givenName":"Robert C","familyName":"Piper","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Tetraspan cargo adaptors usher GPI-anchored proteins into multivesicular bodies"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Neuroscience"},{"subject":"Biotechnology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"}],"contributors":[],"dates":[{"date":"2015-12-12","dateType":"Created"},{"date":"2018-08-31","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1100773","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["27950 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Ubiquitinated membrane proteins are sorted into intralumenal endosomal vesicles on their way for degradation in lysosomes. Here we summarize the discovery of the Cos proteins, which work to organize and segregate ubiquitinated cargo prior to its incorporation into intralumenal vesicles of the multivesicular body (MVB). Importantly, cargoes such as GPI-anchored proteins (GPI-APs) that cannot undergo ubiquitination, rely entirely on Cos proteins for sorting into intralumenal vesicles using the same pathway that depends on ESCRTs and ubiquitin ligases that typical polytopic membrane proteins do. Here we show Cos proteins provide functions as not only adaptor proteins for ubiquitin ligases, but also as cargo carriers that can physically usher a variety of other proteins into the MVB pathway. We then discuss the significance of this new sorting model and the broader implications for this cargo adaptor mechanism, whereby yeast Cos proteins, and their likely animal analogs, provide a ubiquitin sorting signal in \u003ci\u003etrans\u003c/i\u003e to enable sorting of a membrane protein network into intralumenal vesicles.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Tetraspan_cargo_adaptors_usher_GPI_anchored_proteins_into_multivesicular_bodies/1623156","contentUrl":null,"metadataVersion":6,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reaso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