{"data":{"id":"10.6084/m9.figshare.1621235","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1621235","prefix":"10.6084","suffix":"m9.figshare.1621235","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Runqiang Yang","affiliation":[],"nameIdentifiers":[]},{"name":"Qianru Hui","affiliation":[],"nameIdentifiers":[]},{"name":"Zhenxin Gu","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Effects of ABA and CaCl\u003csub\u003e2\u003c/sub\u003e on GABA accumulation in fava bean germinating under hypoxia-NaCl stress"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Science Policy"},{"subject":"Marine Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-12-08","dateType":"Created"},{"date":"2015-12-08","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["727040 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Effects of exogenous abscisic acid (ABA) and CaCl\u003csub\u003e2\u003c/sub\u003e on γ-aminobutyric acid (GABA) accumulation of germinated fava bean under hypoxia-NaCl stress were investigated. Exogenous ABA resulted in the enhancement of glutamate decarboxylase (GAD) and diamine oxidase (DAO) activity as well as GABA content in cotyledon and shoot. CaCl\u003csub\u003e2\u003c/sub\u003e increased both enzyme activities in shoot and GABA content in cotyledon and shoot. ABA downregulated \u003ci\u003eGAD\u003c/i\u003e expression in cotyledon and radicle, while upregulated that in shoot; it also upregulated \u003ci\u003eDAO\u003c/i\u003e expression in each organ. CaCl\u003csub\u003e2\u003c/sub\u003e upregulated \u003ci\u003eGAD\u003c/i\u003e expression in cotyledon, while downregulated that in radicle. However, it upregulated \u003ci\u003eDAO\u003c/i\u003e expression in shoot, downregulated that in radicle. ABA inhibitor fluridon and ethylenediaminetetraacetic acid inhibited GAD and DAO activities significantly so that inhibited GABA accumulation through reducing ABA biosynthesis and chelating Ca\u003csup\u003e2+\u003c/sup\u003e, respectively. However, they upregulated \u003ci\u003eGAD\u003c/i\u003e and \u003ci\u003eDAO\u003c/i\u003e expression in varying degrees. These results indicate that ABA and Ca\u003csup\u003e2+\u003c/sup\u003e participate in GABA biosynthesis in fava bean during germination under hypoxia-NaCl stress.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Effects_of_ABA_and_CaCl_sub_2_sub_on_GABA_accumulation_in_fava_bean_germinating_under_hypoxia_NaCl_stress/1621235","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-12-08T04:31:59.000Z","registered":"2015-12-08T04:32:00.000Z","published":"2015","updated":"2020-09-04T14:00:50.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1621235","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}