{"data":{"id":"10.6084/m9.figshare.1618809","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1618809","prefix":"10.6084","suffix":"m9.figshare.1618809","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Borodkina, Aleksandra V.","nameType":"Personal","givenName":"Aleksandra V.","familyName":"Borodkina","affiliation":[],"nameIdentifiers":[]},{"name":"Shatrova, Alla N.","nameType":"Personal","givenName":"Alla N.","familyName":"Shatrova","affiliation":[],"nameIdentifiers":[]},{"name":"Deryabin, Pavel I.","nameType":"Personal","givenName":"Pavel I.","familyName":"Deryabin","affiliation":[],"nameIdentifiers":[]},{"name":"Grukova, Anastasiya A.","nameType":"Personal","givenName":"Anastasiya A.","familyName":"Grukova","affiliation":[],"nameIdentifiers":[]},{"name":"Nikolsky, Nikolay N.","nameType":"Personal","givenName":"Nikolay N.","familyName":"Nikolsky","affiliation":[],"nameIdentifiers":[]},{"name":"Burova, Elena B.","nameType":"Personal","givenName":"Elena B.","familyName":"Burova","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Tetraploidization or autophagy: the ultimate fate of senescent human endometrial stem cells under ATM or p53 inhibition"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Earth and Environmental Sciences"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-12-04","dateType":"Created"},{"date":"2015-12-08","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["7279864 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Previously we demonstrated that endometrium-derived human mesenchymal stem cells (hMESCs) via activation of the ATM/p53/p21/Rb pathway enter the premature senescence in response to oxidative stress. Down regulation effects of the key components of this signaling pathway, particularly ATM and p53, on a fate of stressed hMESCs have not yet been investigated. In the present study by using the specific inhibitors Ku55933 and Pifithrin-α, we confirmed implication of both ATM and p53 in H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-induced senescence of hMESCs. ATM or p53 down regulation was shown to modulate differently the cellular fate of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-treated hMESCs. ATM inhibition allowed H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-stimulated hMESCs to escape the permanent cell cycle arrest due to loss of the functional ATM/p53/p21/Rb pathway, and induced bypass of mitosis and re-entry into S phase, resulting in tetraploid cells. On the contrary, suppression of the p53 transcriptional activity caused a pronounced cell death of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-treated hMESCs via autophagy induction. The obtained data clearly demonstrate that down regulation of ATM or p53 shifts senescence of human endometrial stem cells towards tetraploidization or autophagy","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Tetraploidization_or_autophagy_the_ultimate_fate_of_senescent_human_endometrial_stem_cells_under_ATM_or_p53_inhibition/1618809","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-12-04T16:40:20.000Z","registered":"2015-12-04T16:40:21.000Z","published":"2015","updated":"2020-09-04T14:02:53.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1618809","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}