{"data":{"id":"10.6084/m9.figshare.1613431","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1613431","prefix":"10.6084","suffix":"m9.figshare.1613431","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Javed, Numan","nameType":"Personal","givenName":"Numan","familyName":"Javed","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Cross reactivity of S. aureus to murine cytokine assays: A source of discrepancy"}],"publisher":"figshare","container":{},"publicationYear":2015,"subjects":[{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2015-11-28","dateType":"Created"},{"date":"2015-12-02","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[],"relatedItems":[],"sizes":["782321 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Staphylococcus aureus is one of the versatile Gram positive bacteria causing a range of diseases. Upon challenge, host immune cells recognize S. aureus and mount diverse immune responses including production of pro-inflammatory cytokines such as IL-1β and TNF-α. These cytokines are important mediators of inflammation which can be detected via various immunological methods such as enzyme linked immunosorbent assay (ELISA). In the current study, we found that a number of clinical isolates as well as laboratory strains of S. aureus exhibited cross reactivity with ELISA antibodies for murine IL-1β and TNF-α assays. This cross reactivity generates exaggerated false positive signals which can be a source of discrepancy for the understanding of real immune responses against S. aureus infection by host immune cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/dataset/Cross_reactivity_of_S_aureus_to_murine_cytokine_assays_A_source_of_discrepancy/1613431","contentUrl":null,"metadataVersion":1,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-12-01T03:26:24.000Z","registered":"2015-12-01T03:26:25.000Z","published":"2015","updated":"2020-09-04T14:18:18.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1613431","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}