{"data":{"id":"10.6084/m9.figshare.1603402","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1603402","prefix":"10.6084","suffix":"m9.figshare.1603402","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Adrivanio Baranoski","affiliation":[],"nameIdentifiers":[]},{"name":"Oliveira, Marcelo Tempesta","nameType":"Personal","givenName":"Marcelo Tempesta","familyName":"Oliveira","affiliation":[],"nameIdentifiers":[]},{"name":"Semprebon, Simone Cristine","nameType":"Personal","givenName":"Simone Cristine","familyName":"Semprebon","affiliation":[],"nameIdentifiers":[]},{"name":"Andressa Megumi Niwa","affiliation":[],"nameIdentifiers":[]},{"name":"Ribeiro, Lúcia Regina","nameType":"Personal","givenName":"Lúcia Regina","familyName":"Ribeiro","affiliation":[],"nameIdentifiers":[]},{"name":"Mantovani, Mário Sérgio","nameType":"Personal","givenName":"Mário Sérgio","familyName":"Mantovani","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Effects of sulfated and non-sulfated \u003cb\u003eβ\u003c/b\u003e-glucan extracted from \u003ci\u003eAgaricus brasiliensis\u003c/i\u003e in breast adenocarcinoma cells – MCF-7"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Cancer"},{"subject":"Plant Biology"}],"contributors":[],"dates":[{"date":"2015-11-16","dateType":"Created"},{"date":"2017-04-26","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/15376516.2015.1043762","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["429220 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The β-glucans (β-G) are polysaccharides produced by various organisms, and sulfation of β-G renders them more soluble. With the objective to assess the effects of sulfated and non-sulfated β-G extracted from \u003ci\u003eAgaricus brasiliensis\u003c/i\u003e in MCF-7 cells, assays were used to evaluate cytotoxicity, genotoxicity, cell proliferation and mRNA expression. The sulfated and non-sulfated β-G showed dose-dependent cytotoxicity at concentrations of 5 and 10 μg/mL, by the MTT assay. However, only cytotoxicity was observed after 24 h by the Red Neutral test for sulfated β-G, with no genotoxicity for either β-G in comet assay. Proliferation was decreased only at 72 h at a concentration of 100 μg/mL of sulfated β-G. Treatment with 5 μg/mL of sulfated β-G for 6 h reduced the expression of pro-apoptotic genes and stress signaling genes, cell cycle arrest, damage and cell migration. The 5 μg/mL of non-sulfated β-G for 6 h reduced the expression of the stress response gene and signaling damage. These results indicate that the cytotoxicity in the MTT is not cell death, and that, in general, sulfated β-G have greater cytotoxicity compared to non-sulfated β-G.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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