{"data":{"id":"10.6084/m9.figshare.1597994","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1597994","prefix":"10.6084","suffix":"m9.figshare.1597994","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Kohtaro Miyazawa","affiliation":[],"nameIdentifiers":[]},{"name":"Okada, Hiroyuki","nameType":"Personal","givenName":"Hiroyuki","familyName":"Okada","affiliation":[],"nameIdentifiers":[]},{"name":"Masujin, Kentaro","nameType":"Personal","givenName":"Kentaro","familyName":"Masujin","affiliation":[],"nameIdentifiers":[]},{"name":"Iwamaru, Yoshifumi","nameType":"Personal","givenName":"Yoshifumi","familyName":"Iwamaru","affiliation":[],"nameIdentifiers":[]},{"name":"Yokoyama, Takashi","nameType":"Personal","givenName":"Takashi","familyName":"Yokoyama","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Infectivity-associated PrP\u003csup\u003eSc\u003c/sup\u003e and disease duration-associated PrP\u003csup\u003eSc\u003c/sup\u003e of mouse BSE prions"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Medicine"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Neuroscience"},{"subject":"Ecology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Science Policy"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2015-11-10","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/19336896.2015.1111507","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["122237 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Disease-related prion protein (PrP\u003csup\u003eSc\u003c/sup\u003e), which is a structural isoform of the host-encoded cellular prion protein, is thought to be a causative agent of transmissible spongiform encephalopathies. However, the specific role of PrP\u003csup\u003eSc\u003c/sup\u003e in prion pathogenesis and its relationship to infectivity remain controversial. A time-course study of prion-affected mice was conducted, which showed that the prion infectivity was not simply proportional to the amount of PrP\u003csup\u003eSc\u003c/sup\u003e in the brain. Centrifugation (20,000 ×\u003ci\u003eg\u003c/i\u003e) of the brain homogenate showed that most of the PrP\u003csup\u003eSc\u003c/sup\u003e was precipitated into the pellet, and the supernatant contained only a slight amount of PrP\u003csup\u003eSc\u003c/sup\u003e. Interestingly, mice inoculated with the obtained supernatant showed incubation periods that were approximately 15 d longer than those of mice inoculated with the crude homogenate even though both inocula contained almost the same infectivity. Our results suggest that a small population of fine PrP\u003csup\u003eSc\u003c/sup\u003e may be responsible for prion infectivity and that large, aggregated PrP\u003csup\u003eSc\u003c/sup\u003e may contribute to determining prion disease duration.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Infectivity_associated_PrP_sup_Sc_sup_and_disease_duration_associ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