{"data":{"id":"10.6084/m9.figshare.1591899","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1591899","prefix":"10.6084","suffix":"m9.figshare.1591899","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Bansal, Reema","givenName":"Reema","familyName":"Bansal","affiliation":[],"nameIdentifiers":[]},{"name":"Liermann, Laura J.","givenName":"Laura J.","familyName":"Liermann","affiliation":[],"nameIdentifiers":[]},{"name":"Stanley, Bruce A.","givenName":"Bruce A.","familyName":"Stanley","affiliation":[],"nameIdentifiers":[]},{"name":"Brantley, Susan L.","givenName":"Susan L.","familyName":"Brantley","affiliation":[],"nameIdentifiers":[]},{"name":"Tien, Ming","givenName":"Ming","familyName":"Tien","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Characterization and Proteomic Analysis of \u003ci\u003eGeobacter sulfurreducens\u003c/i\u003e PCA under Long-Term Electron-Donor Starvation"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2024,"subjects":[{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biotechnology"},{"subject":"Ecology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Developmental Biology"},{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2024-10-17","dateType":"Created"},{"date":"2024-11-01","dateType":"Updated"},{"date":"2024","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/01490451.2015.1099765","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1565882 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The natural environment of \u003ci\u003eGeobacter sulfurreducens\u003c/i\u003e is oligotrophic and described as limiting in both electron donors and terminal electron acceptors (TEA). In previous studies we examined the effects of long-term TEA (fumarate) limitation, and in this study we examine long-term batch cultures under limiting electron donor. The microorganism survived under long-term electron donor (acetate) starvation, maintaining a stable population of ∼1–2 × 10\u003csup\u003e8\u003c/sup\u003e cells mL\u003csup\u003e−1\u003c/sup\u003e for \u0026gt;650 days. Proteins that varied in abundance with a high level of statistical significance (\u003ci\u003ep\u003c/i\u003e \u0026lt; 0.05) for stages between mid-log to survival phase (acetate starved) were identified using iTRAQ based mass spectroscopy. The most highly represented proteins that significantly increased in level in the survival phase cells are generally membrane-associated and are involved in energy metabolism and protein fate. These results document that changes in the outer and cytoplasmic membranes may help \u003ci\u003eG. sulfurreducens\u003c/i\u003e survive during starvation through detection and transport of nutrients into the cell. A sizeable portion of the identified proteins with unknown or hypothetical function further suggest that much of the biological process involved in survival have yet to be fully understood. \u003ci\u003eGeobacter sulfurreducens\u003c/i\u003e was also able to survive under long-term TEA-starvation conditions with ferric citrate as TEA and maintained a stable population of 1.5–3 × 10\u003csup\u003e7\u003c/sup\u003e cells mL\u003csup\u003e−1\u003c/sup\u003e for \u0026gt;650 days. We also found that survival phase cells from fumarate-limiting conditions were able to quickly resuscitate and reduce metal such as ferric iron as compared to the mid-log phase cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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