{"data":{"id":"10.6084/m9.figshare.1591140","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1591140","prefix":"10.6084","suffix":"m9.figshare.1591140","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Salawu, Musa O.","nameType":"Personal","givenName":"Musa O.","familyName":"Salawu","affiliation":[],"nameIdentifiers":[]},{"name":"Oloyede, Hussein O. B.","nameType":"Personal","givenName":"Hussein O. B.","familyName":"Oloyede","affiliation":[],"nameIdentifiers":[]},{"name":"Temidayo A. Oladiji","affiliation":[],"nameIdentifiers":[]},{"name":"Yakubu, Musa T.","nameType":"Personal","givenName":"Musa T.","familyName":"Yakubu","affiliation":[],"nameIdentifiers":[]},{"name":"Aliyu O. Amuzat","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Hemolymph coagulation and phenoloxidase activity in \u003ci\u003eUca tangeri\u003c/i\u003e induced by \u003ci\u003eEscherichia coli\u003c/i\u003e endotoxin"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Physiology"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Science Policy"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Cancer"},{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2015-11-02","dateType":"Created"},{"date":"2016-06-16","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/1547691x.2015.1096983","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["77963 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eUca tangeri\u003c/i\u003e is a marine fiddler crab found commonly in the West African coast and is often exposed to Gram-negative pathogens upon injury. The aim of this study was to document the patterns of endotoxin-induced protein coagulation and phenoloxidase (PO) activity in hemolymph fractions of \u003ci\u003eUca tangeri\u003c/i\u003e. Hemolymph from live crabs was obtained by carapace puncture, pooled. and then separated into plasma, hemocyte Lysate (HL), hemocyte lysate supernatant (HLS) and hemocyte lysate debris (HLD). The effect of \u003ci\u003eEscherichia coli\u003c/i\u003e (O1111:B4) endotoxin and calcium ion (Ca\u003csup\u003e2+\u003c/sup\u003e) on protein coagulation in the presence/absence of endotoxin and the endotoxin dose-dependence of coagulation and PO activity were each studied in the plasma, HL, HLS and HLD. The results showed Ca\u003csup\u003e2+\u003c/sup\u003e was required to induce coagulation, and was endotoxin concentration-dependent in the plasma. PO activity was highest in the HLS but PO specific activity was highest in HLD. PO activity remained relatively constant with increased LPS concentration in the range studied 0–10 EU/ml. From the data we conclude that endotoxin-induced protein coagulation occurs in the plasma alone and might be mediated by trans-glutaminases, while PO activity is localized inside hemocytes and cell membranes in \u003ci\u003eUca tangeri\u003c/i\u003e.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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