{"data":{"id":"10.6084/m9.figshare.1576400","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1576400","prefix":"10.6084","suffix":"m9.figshare.1576400","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Han, Jae Woong","nameType":"Personal","givenName":"Jae Woong","familyName":"Han","affiliation":[],"nameIdentifiers":[]},{"name":"Jae-Kyo Jeong","affiliation":[],"nameIdentifiers":[]},{"name":"Sangiliyandi Gurunathan","affiliation":[],"nameIdentifiers":[]},{"name":"Yun-Jung Choi","affiliation":[],"nameIdentifiers":[]},{"name":"Joydeep Das","affiliation":[],"nameIdentifiers":[]},{"name":"Deug-Nam Kwon","affiliation":[],"nameIdentifiers":[]},{"name":"Ssang-Goo Cho","affiliation":[],"nameIdentifiers":[]},{"name":"Chankyu Park","affiliation":[],"nameIdentifiers":[]},{"name":"Seo, Han Geuk","nameType":"Personal","givenName":"Han Geuk","familyName":"Seo","affiliation":[],"nameIdentifiers":[]},{"name":"Jin-Ki Park","affiliation":[],"nameIdentifiers":[]},{"name":"Jin-Hoi Kim","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Male- and female-derived somatic and germ cell-specific toxicity of silver nanoparticles in mouse"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Developmental Biology"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2015-10-15","dateType":"Created"},{"date":"2016-07-06","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/17435390.2015.1073396","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["16382171 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Silver nanoparticles (AgNPs) are widely used as an antibiotic agent in textiles, wound dressings, medical devices, and appliances such as refrigerators and washing machines. The increasing use of AgNPs has raised concerns about their potential risks to human health. Therefore, this study was aimed to determine the impact of AgNPs in germ cell specific complications in mice. The administration of AgNPs results in toxicity in mice; however, a more detailed understanding of the effects of AgNPs on germ cells remains poorly understood. Here, we demonstrate the effects of AgNPs (20 nm in diameter) in a mouse Sertoli and granulosa cells \u003ci\u003ein vitro\u003c/i\u003e, and in male and female mice \u003ci\u003ein vivo\u003c/i\u003e. Soluble silver ion (Ag\u003csup\u003e+\u003c/sup\u003e)-treated cells were used as a positive control. We found that excessive AgNP-treated cells exhibited cytotoxicity, the formation of autophagosomes and autolysosomes in Sertoli cells. Furthermore, an increase in mitochondrial-mediated apoptosis by cytochrome \u003ci\u003ec\u003c/i\u003e release from mitochondria due to translocation of Bax to mitochondria was observed. In \u003ci\u003ein vivo\u003c/i\u003e studies, the expression of pro-inflammatory cytokines, including tumor necrosis factor α, interferon-γ, −6, −1β, and monocyte chemoattractant protein-1 were significantly increased (\u003ci\u003ep\u003c/i\u003e","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjEvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLjE1NzY0MDA8L2lkZW50aWZpZXI+CiAgPGNyZWF0b3JzPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5KYWUgV29vbmcgSGFuPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+SmFlLUt5byBKZW9uZzwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlNhbmdpbGl5YW5kaSBHdXJ1bmF0aGFuPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+WXVuLUp1bmcgQ2hvaTwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkpveWRlZXAgRGFzPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+RGV1Zy1OYW0gS3dvbjwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlNzYW5nLUdvbyBDaG88L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5DaGFua3l1IFBhcms8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5IYW4gR2V1ayBTZW88L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5KaW4tS2kgUGFyazwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkppbi1Ib2kgS2ltPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICA8L2NyZWF0b3JzPgogIDx0aXRsZXM+CiAgICA8dGl0bGU+TWFsZS0gYW5kIGZlbWFsZS1kZXJpdmVkIHNvbWF0aWMgYW5kIGdlcm0gY2VsbC1zcGVjaWZpYyB0b3hpY2l0eSBvZiBzaWx2ZXIgbmFub3BhcnRpY2xlcyBpbiBtb3VzZTwvdGl0bGU+CiAgPC90aXRsZXM+CiAgPGRlc2NyaXB0aW9ucz4KICAgIDxkZXNjcmlwdGlvbiBkZXNjcmlwdGlvblR5cGU9IkFic3RyYWN0Ij4mbHQ7cCZndDtTaWx2ZXIgbmFub3BhcnRpY2xlcyAoQWdOUHMpIGFyZSB3aWRlbHkgdXNlZCBhcyBhbiBhbnRpYmlvdGljIGFnZW50IGluIHRleHRpbGVzLCB3b3VuZCBkcmVzc2luZ3MsIG1lZGljYWwgZGV2aWNlcywgYW5kIGFwcGxpYW5jZXMgc3VjaCBhcyByZWZyaWdlcmF0b3JzIGFuZCB3YXNoaW5nIG1hY2hpbmVzLiBUaGUgaW5jcmVhc2luZyB1c2Ugb2YgQWdOUHMgaGFzIHJhaXNlZCBjb25jZXJucyBhYm91dCB0aGVpciBwb3RlbnRpYWwgcmlza3MgdG8gaHVtYW4gaGVhbHRoLiBUaGVyZWZvcmUsIHRoaXMgc3R1ZHkgd2FzIGFpbWVkIHRvIGRldGVybWluZSB0aGUgaW1wYWN0IG9mIEFnTlBzIGluIGdlcm0gY2VsbCBzcGVjaWZpYyBjb21wbGljYXRpb25zIGluIG1pY2UuIFRoZSBhZG1pbmlzdHJhdGlvbiBvZiBBZ05QcyByZXN1bHRzIGluIHRveGljaXR5IGluIG1pY2U7IGhvd2V2ZXIsIGEgbW9yZSBkZXRhaWxlZCB1bmRlcnN0YW5kaW5nIG9mIHRoZSBlZmZlY3RzIG9mIEFnTlBzIG9uIGdlcm0gY2VsbHMgcmVtYWlucyBwb29ybHkgdW5kZXJzdG9vZC4gSGVyZSwgd2UgZGVtb25zdHJhdGUgdGhlIGVmZmVjdHMgb2YgQWdOUHMgKDIw4oCJbm0gaW4gZGlhbWV0ZXIpIGluIGEgbW91c2UgU2VydG9saSBhbmQgZ3JhbnVsb3NhIGNlbGxzICZsdDtpJmd0O2luIHZpdHJvJmx0Oy9pJmd0OywgYW5kIGluIG1hbGUgYW5kIGZlbWFsZSBtaWNlICZsdDtpJmd0O2luIHZpdm8mbHQ7L2kmZ3Q7LiBTb2x1YmxlIHNpbHZlciBpb24gKEFnJmx0O3N1cCZndDsrJmx0Oy9zdXAmZ3Q7KS10cmVhdGVkIGNlbGxzIHdlcmUgdXNlZCBhcyBhIHBvc2l0aXZlIGNvbnRyb2wuIFdlIGZvdW5kIHRoYXQgZXhjZXNzaXZlIEFnTlAtdHJlYXRlZCBjZWxscyBleGhpYml0ZWQgY3l0b3RveGljaXR5LCB0aGUgZm9ybWF0aW9uIG9mIGF1dG9waGFnb3NvbWVzIGFuZCBhdXRvbHlzb3NvbWVzIGluIFNlcnRvbGkgY2VsbHMuIEZ1cnRoZXJtb3JlLCBhbiBpbmNyZWFzZSBpbiBtaXRvY2hvbmRyaWFsLW1lZGlhdGVkIGFwb3B0b3NpcyBieSBjeXRvY2hyb21lICZsdDtpJmd0O2MmbHQ7L2kmZ3Q7IHJlbGVhc2UgZnJvbSBtaXRvY2hvbmRyaWEgZHVlIHRvIHRyYW5zbG9jYXRpb24gb2YgQmF4IHRvIG1pdG9jaG9uZHJpYSB3YXMgb2JzZXJ2ZWQuIEluICZsdDtpJmd0O2luIHZpdm8mbHQ7L2kmZ3Q7IHN0dWRpZXMsIHRoZSBleHByZXNzaW9uIG9mIHByby1pbmZsYW1tYXRvcnkgY3l0b2tpbmVzLCBpbmNsdWRpbmcgdHVtb3IgbmVjcm9zaXMgZmFjdG9yIM6xLCBpbnRlcmZlcm9uLc6zLCDiiJI2LCDiiJIxzrIsIGFuZCBtb25vY3l0ZSBjaGVtb2F0dHJhY3RhbnQgcHJvdGVpbi0xIHdlcmUgc2lnbmlmaWNhbnRseSBpbmNyZWFzZWQgKCZsdDtpJmd0O3AmbHQ7L2kmZ3Q74oCJJmx0O+KAiTAuMDUpLiBIaXN0b3BhdGhvbG9naWNhbCBhbmFseXNpcyBvZiBBZ05QLXRyZWF0ZWQgbWljZSBzaG93cyB0aGF0IGEgc2lnbmlmaWNhbnQgbG9zcyBvZiBtYWxlIGFuZCBmZW1hbGUgZ2VybSBjZWxscy4gVGFrZW4gdG9nZXRoZXIsIHRoZXNlIGRhdGEgc3VnZ2VzdCB0aGF0IEFnTlBzIHdpdGggYW4gYXZlcmFnZSBzaXplIG9mIDIw4oCJbm0gaGF2ZSBuZWdhdGl2ZSBpbXBhY3Qgb24gdGhlIHJlcHJvZHVjdGlvbi4mbHQ7L3AmZ3Q7PC9kZXNjcmlwdGlvbj4KICA8L2Rlc2NyaXB0aW9ucz4KICA8c3ViamVjdHM+CiAgICA8c3ViamVjdD5NZWRpY2luZTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkNlbGwgQmlvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PlBoeXNpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdCBzY2hlbWVVUkk9Imh0dHA6Ly93d3cuYWJzLmdvdi5hdS9hdXNzdGF0cy9hYnNALm5zZi8wLzZCQjQyN0FCOTY5NkMyMjVDQTI1NzQxODAwMDQ0NjNFIiBzdWJqZWN0U2NoZW1lPSJGT1IiPjU5OTk5IEVudmlyb25tZW50YWwgU2NpZW5jZXMgbm90IGVsc2V3aGVyZSBjbGFzc2lmaWVkPC9zdWJqZWN0PgogICAgPHN1YmplY3Qgc2NoZW1lVVJJPSJodHRwOi8vd3d3LmFicy5nb3YuYXUvYXVzc3RhdHMvYWJzQC5uc2YvMC82QkI0MjdBQjk2OTZDMjI1Q0EyNTc0MTgwMDA0NDYzRSIgc3ViamVjdFNjaGVtZT0iRk9SIj4zOTk5OSBDaGVtaWNhbCBTY2llbmNlcyBub3QgZWxzZXdoZXJlIGNsYXNzaWZpZWQ8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5JbW11bm9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Qgc2NoZW1lVVJJPSJodHRwOi8vd3d3LmFicy5nb3YuYXUvYXVzc3RhdHMvYWJzQC5uc2YvMC82QkI0MjdBQjk2OTZDMj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