{"data":{"id":"10.6084/m9.figshare.1571362","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1571362","prefix":"10.6084","suffix":"m9.figshare.1571362","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Li, Mo","nameType":"Personal","givenName":"Mo","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Chunjing Bian","affiliation":[],"nameIdentifiers":[]},{"name":"Xiaochun Yu","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Poly(ADP-ribosyl)ation is recognized by ECT2 during mitosis"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2014,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Astronomy, Astrophysics, Space Science"},{"subject":"Neuroscience"},{"subject":"Cell Biology"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-09","dateType":"Created"},{"date":"2015-10-09","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15384101.2014.947197","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["863033 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Poly(ADP-ribosyl)ation is an unique posttranslational modification and required for spindle assembly and function during mitosis. However, the molecular mechanism of poly(ADP-ribose) (PAR) in mitosis remains elusive. Here, we show the evidence that PAR is recognized by ECT2, a key guanine nucleotide exchange factor in mitosis. The BRCT domain of ECT2 directly binds to PAR both \u003ci\u003ein vitro\u003c/i\u003e and \u003ci\u003ein vivo\u003c/i\u003e. We further found that α-tubulin is PARylated during mitosis. PARylation of α-tubulin is recognized by ECT2 and recruits ECT2 to mitotic spindle for completing mitosis. Taken together, our study reveals a novel mechanism by which PAR regulates mitosis.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Poly_ADP_ribosyl_ation_is_recognized_by_ECT2_during_mitosis/1571362","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-10-09T21:48:34.000Z","registered":"2015-10-09T21:48:35.000Z","published":"2014","updated":"2020-09-04T15:01:16.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1571362","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}