{"data":{"id":"10.6084/m9.figshare.1571354","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1571354","prefix":"10.6084","suffix":"m9.figshare.1571354","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Fengying Liu","affiliation":[],"nameIdentifiers":[]},{"name":"Wald, Diana","nameType":"Personal","givenName":"Diana","familyName":"Wald","affiliation":[],"nameIdentifiers":[]},{"name":"Rothermel, Ulrike","nameType":"Personal","givenName":"Ulrike","familyName":"Rothermel","affiliation":[],"nameIdentifiers":[]},{"name":"Ashery-Padan, Ruth","nameType":"Personal","givenName":"Ruth","familyName":"Ashery-Padan","affiliation":[],"nameIdentifiers":[]},{"name":"Hermann-Josef Gröne","affiliation":[],"nameIdentifiers":[]},{"name":"Izraeli, Shai","nameType":"Personal","givenName":"Shai","familyName":"Izraeli","affiliation":[],"nameIdentifiers":[]},{"name":"Krämer, Alwin","nameType":"Personal","givenName":"Alwin","familyName":"Krämer","affiliation":[],"nameIdentifiers":[]},{"name":"Ahuvit David","affiliation":[],"nameIdentifiers":[]},{"name":"Tibelius, Alexandra","nameType":"Personal","givenName":"Alexandra","familyName":"Tibelius","affiliation":[],"nameIdentifiers":[]},{"name":"Vulprecht, Julia","nameType":"Personal","givenName":"Julia","familyName":"Vulprecht","affiliation":[],"nameIdentifiers":[]},{"name":"Ohana, Reut","nameType":"Personal","givenName":"Reut","familyName":"Ohana","affiliation":[],"nameIdentifiers":[]},{"name":"Seitel, Alexander","nameType":"Personal","givenName":"Alexander","familyName":"Seitel","affiliation":[],"nameIdentifiers":[]},{"name":"Metzger, Jasmin","nameType":"Personal","givenName":"Jasmin","familyName":"Metzger","affiliation":[],"nameIdentifiers":[]},{"name":"Hans-Peter Meinzer","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Lack of centrioles and primary cilia in \u003ci\u003eSTIL\u003csup\u003e−/−\u003c/sup\u003e\u003c/i\u003e mouse embryos"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2014,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"},{"subject":"Marine Biology"},{"subject":"Developmental Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Neuroscience"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-09","dateType":"Created"},{"date":"2015-10-09","dateType":"Updated"},{"date":"2014","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.4161/15384101.2014.946830","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["206798523 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Although most animal cells contain centrosomes, consisting of a pair of centrioles, their precise contribution to cell division and embryonic development is unclear. Genetic ablation of \u003ci\u003eSTIL\u003c/i\u003e, an essential component of the centriole replication machinery in mammalian cells, causes embryonic lethality in mice around mid gestation associated with defective Hedgehog signaling. Here, we describe, by focused ion beam scanning electron microscopy, that \u003ci\u003eSTIL\u003csup\u003e−/−\u003c/sup\u003e\u003c/i\u003e mouse embryos do not contain centrioles or primary cilia, suggesting that these organelles are not essential for mammalian development until mid gestation. We further show that the lack of primary cilia explains the absence of Hedgehog signaling in \u003ci\u003eSTIL\u003csup\u003e−/−\u003c/sup\u003e\u003c/i\u003e cells. Exogenous re-expression of STIL or STIL microcephaly mutants compatible with human survival, induced non-templated, \u003ci\u003ede novo\u003c/i\u003e generation of centrioles in \u003ci\u003eSTIL\u003csup\u003e−/−\u003c/sup\u003e\u003c/i\u003e cells. Thus, while the abscence of centrioles is compatible with mammalian gastrulation, lack of centrioles and primary cilia impairs Hedgehog signaling and further embryonic development.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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