{"data":{"id":"10.6084/m9.figshare.1569746","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569746","prefix":"10.6084","suffix":"m9.figshare.1569746","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Dubucquoi, Sylvain","nameType":"Personal","givenName":"Sylvain","familyName":"Dubucquoi","affiliation":[],"nameIdentifiers":[]},{"name":"Duban-Deweer, Sophie","nameType":"Personal","givenName":"Sophie","familyName":"Duban-Deweer","affiliation":[],"nameIdentifiers":[]},{"name":"Prin, Lionel","nameType":"Personal","givenName":"Lionel","familyName":"Prin","affiliation":[],"nameIdentifiers":[]},{"name":"Lefranc, Didier","nameType":"Personal","givenName":"Didier","familyName":"Lefranc","affiliation":[],"nameIdentifiers":[]},{"name":"Proust-Lemoine, Emmanuelle","nameType":"Personal","givenName":"Emmanuelle","familyName":"Proust-Lemoine","affiliation":[],"nameIdentifiers":[]},{"name":"E. Helen Kemp","affiliation":[],"nameIdentifiers":[]},{"name":"Ryndak, Amélie","nameType":"Personal","givenName":"Amélie","familyName":"Ryndak","affiliation":[],"nameIdentifiers":[]},{"name":"Lefèvre-Dutoit, Virginie","nameType":"Personal","givenName":"Virginie","familyName":"Lefèvre-Dutoit","affiliation":[],"nameIdentifiers":[]},{"name":"Bellart, Marine","nameType":"Personal","givenName":"Marine","familyName":"Bellart","affiliation":[],"nameIdentifiers":[]},{"name":"Saugier-Véber, Pascale","nameType":"Personal","givenName":"Pascale","familyName":"Saugier-Véber","affiliation":[],"nameIdentifiers":[]},{"name":"Jean-Louis Wémeau","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Serological proteome analysis reveals new specific biases in the IgM and IgG autoantibody repertoires in autoimmune polyendocrine syndrome type 1"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"60506 Virology","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Medicine"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2015-10-08","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/08916934.2015.1077230","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["3183219 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eObjective\u003c/i\u003e: Autoimmune polyendocrine syndrome type 1 (APS 1) is caused by mutations in the \u003ci\u003eAIRE\u003c/i\u003e gene that induce intrathymic T-cell tolerance breakdown, which results in tissue-specific autoimmune diseases. \u003ci\u003eDesign\u003c/i\u003e: To evaluate the effect of a well-defined T-cell repertoire impairment on humoral self-reactive fingerprints, comparative serum self-IgG and self-IgM reactivities were analyzed using both one- and two-dimensional western blotting approaches against a broad spectrum of peripheral tissue antigens. \u003ci\u003eMethods\u003c/i\u003e: Autoantibody patterns of APS 1 patients were compared with those of subjects affected by other autoimmune endocrinopathies (OAE) and healthy controls. \u003ci\u003eResults\u003c/i\u003e: Using a Chi-square test, significant changes in the Ab repertoire were found when intergroup patterns were compared. A singular distortion of both serum self-IgG and self-IgM repertoires was noted in APS 1 patients. The molecular characterization of these antigenic targets was conducted using a proteomic approach. In this context, autoantibodies recognized more significantly either tissue-specific antigens, such as pancreatic amylase, pancreatic triacylglycerol lipase and pancreatic regenerating protein 1α, or widely distributed antigens, such as peroxiredoxin-2, heat shock cognate 71-kDa protein and aldose reductase. As expected, a well-defined self-reactive T-cell repertoire impairment, as described in APS 1 patients, affected the tissue-specific self-IgG repertoire. Interestingly, discriminant IgM reactivities targeting both tissue-specific and more widely expressed antigens were also specifically observed in APS 1 patients. Using recombinant targets, we observed that post translational modifications of these specific antigens impacted upon their recognition. \u003ci\u003eConclusions\u003c/i\u003e: The data suggest that T-cell-dependent but also T-cell-independent mechanisms are involved in the dynamic evolution of autoimmunity in APS 1.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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