{"data":{"id":"10.6084/m9.figshare.1569533.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569533.v1","prefix":"10.6084","suffix":"m9.figshare.1569533.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Kang, Keren","nameType":"Personal","givenName":"Keren","familyName":"Kang","affiliation":[],"nameIdentifiers":[]},{"name":"Peidian Wu","affiliation":[],"nameIdentifiers":[]},{"name":"Wenmei Li","affiliation":[],"nameIdentifiers":[]},{"name":"Shixing Tang","affiliation":[],"nameIdentifiers":[]},{"name":"Jihua Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Xiaochun Luo","affiliation":[],"nameIdentifiers":[]},{"name":"Mingquan Xie","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Evaluation of a newly developed quantitative heart-type fatty acid binding protein assay based on fluorescence immunochromatography using specific monoclonal antibodies"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Cardiology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"},{"subject":"Cancer"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Biotechnology"},{"subject":"Cell Biology"},{"subject":"Engineering"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2016-01-20","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/00365513.2015.1054870","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1569533","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["7803431 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003cb\u003e\u003ci\u003eObjectives.\u003c/i\u003e\u003c/b\u003e To develop a rapid, sensitive and specific assay for quantification of serum heart-type fatty acid binding protein (H-FABP) based on immunofluorescence of specific monoclonal antibodies. \u003cb\u003e\u003ci\u003eDesign and Methods.\u003c/i\u003e\u003c/b\u003e We generated novel H-FABP-directed monoclonal antibodies by cloning of spleen cells of mice immunized with H-FABP. Epitopes were mapped and antigen affinity was assessed by surface plasmon resonance (SPR). The H-FABP specific monoclonal antibodies were coupled to fluorescent beads and sprayed onto a nitrocellulose membrane facilitating quantification of H-FABP by immunofluorescence. Reagent cross-reactivity, interference resistance, accuracy and sensitivity were examined. A total of 103 clinical samples were used to compare the sensitivity and specificity of the new assay to a commercially available Randox kit. \u003cb\u003e\u003ci\u003eResults.\u003c/i\u003e\u003c/b\u003e This new assay could be finished within 15 min, with sensitivity reaching 1 ng/ml. In a trial of 103 clinical serum samples, the new testing kit results were highly correlated with those from the Randox kit (R\u003csup\u003e2\u003c/sup\u003e = 0.9707). Using the Randox kit as the reference kit, the sensitivity of the new assay was 98.25%, and specificity was 100%. \u003cb\u003e\u003ci\u003eConclusions.\u003c/i\u003e\u003c/b\u003e An immunofluorescence-based H-FABP assay employing novel monoclonal antibodies could rapidly, specifically and sensitively detect H-FABP in serum samples, providing an effective method for rapid clinical assessment of H-FABP index in the clinic.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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