{"data":{"id":"10.6084/m9.figshare.1569506","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569506","prefix":"10.6084","suffix":"m9.figshare.1569506","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Govindaraj, Vijayakumar","nameType":"Personal","givenName":"Vijayakumar","familyName":"Govindaraj","affiliation":[],"nameIdentifiers":[]},{"name":"A. Jagannadha Rao","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Comparative proteomic analysis of primordial follicles from ovaries of immature and aged rats"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Developmental Biology"},{"subject":"Mathematics"},{"subject":"FOS: Mathematics","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Mathematics","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences"},{"subject":"Chemistry"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Genetics"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/19396368.2015.1077903","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["2005995 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Age related decline in reproductive performance in women is well documented and apoptosis has been considered as one of the reasons for the decline of primordial follicle reserve. Recently we observed a decline in the efficiency of DNA repair ability in aged rat primordial follicles as demonstrated by decreased mRNA levels of DNA repair genes \u003ci\u003eBRCA1 \u003c/i\u003eand\u003ci\u003e H2AX\u003c/i\u003e. In the present study, a two-dimensional electrophoresis (2DE) proteomic approach was employed to identify differentially expressed proteins in primordial follicles isolated from ovaries of immature (∼20 days) and aged (∼400–450 days) rats. Using MALDI-TOF/TOF MS, we identified 13 differentially expressed proteins 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