{"data":{"id":"10.6084/m9.figshare.1569495","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569495","prefix":"10.6084","suffix":"m9.figshare.1569495","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Suzuki, Kazushi","nameType":"Personal","givenName":"Kazushi","familyName":"Suzuki","affiliation":[],"nameIdentifiers":[]},{"name":"Shimizu, Mari","nameType":"Personal","givenName":"Mari","familyName":"Shimizu","affiliation":[],"nameIdentifiers":[]},{"name":"Sasaki, Naomi","nameType":"Personal","givenName":"Naomi","familyName":"Sasaki","affiliation":[],"nameIdentifiers":[]},{"name":"Chisana Ogawa","affiliation":[],"nameIdentifiers":[]},{"name":"Minami, Haruka","nameType":"Personal","givenName":"Haruka","familyName":"Minami","affiliation":[],"nameIdentifiers":[]},{"name":"Hayuki Sugimoto","affiliation":[],"nameIdentifiers":[]},{"name":"Watanabe, Takeshi","nameType":"Personal","givenName":"Takeshi","familyName":"Watanabe","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Regulation of the chitin degradation and utilization system by the ChiX small RNA in \u003ci\u003eSerratia marcescens\u003c/i\u003e 2170"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Evolutionary Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2015-12-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2015.1083399","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["608768 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eSerratia marcescens\u003c/i\u003e 2170 produces three different types of chitinases and chitin-binding protein CBP21. We found that transposon insertion into the 5′ untranslated region (5′ UTR) of \u003ci\u003echiPQ\u003c/i\u003e-\u003ci\u003ectb\u003c/i\u003e led to defective chitinase and CBP21 production. ChiX small RNA possessed the complementary sequence of the 5′ UTRs of the \u003ci\u003echiPQ\u003c/i\u003e-\u003ci\u003ectb\u003c/i\u003e and \u003ci\u003echiR\u003c/i\u003e and repressed the expression of \u003ci\u003echiP\u003c/i\u003e and \u003ci\u003echiR\u003c/i\u003e. ChiX was detected in a medium containing glucose, glycerol, GlcNAc, and (GlcNAc)\u003csub\u003e2\u003c/sub\u003e, but the expression of both \u003ci\u003echiP\u003c/i\u003e and \u003ci\u003echiR\u003c/i\u003e was only observed in a medium containing (GlcNAc)\u003csub\u003e2\u003c/sub\u003e. ∆\u003ci\u003echiX\u003c/i\u003e mutant produced chitinases, CBP21, and chitobiase without induction. \u003ci\u003echiP\u003c/i\u003e transcripts were more abundant than those of \u003ci\u003echiR\u003c/i\u003e or \u003ci\u003echiX\u003c/i\u003e in a medium containing (GlcNAc)\u003csub\u003e2\u003c/sub\u003e. These results suggest that the constitutively expressed ChiX binds to the highly abundant \u003ci\u003echiP\u003c/i\u003e 5′ UTR, thereby leading to the induction of \u003ci\u003echiR\u003c/i\u003e mRNA translation and the subsequent expression of chitinases and CBP21.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Regulation_of_the_chitin_degradation_and_utilization_system_by_the_ChiX_small_RNA_in_i_Serratia_marcescens_i_2170/1569495","contentUrl":null,"metadataVersion":8,"schemaVersion":"http://datacite.org/schema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