{"data":{"id":"10.6084/m9.figshare.1569452","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569452","prefix":"10.6084","suffix":"m9.figshare.1569452","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Simpson-Lavy, Kobi J","nameType":"Personal","givenName":"Kobi J","familyName":"Simpson-Lavy","affiliation":[],"nameIdentifiers":[]},{"name":"Zenvirth, Drora","nameType":"Personal","givenName":"Drora","familyName":"Zenvirth","affiliation":[],"nameIdentifiers":[]},{"name":"Brandeis, Michael","nameType":"Personal","givenName":"Michael","familyName":"Brandeis","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Phosphorylation and dephosphorylation regulate APC/C\u003csup\u003eCdh1\u003c/sup\u003e substrate degradation"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"Molecular Biology"},{"subject":"Pharmacology"},{"subject":"Cancer"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1078036","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["24688434 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The Anaphase Promoting Complex/Cyclosome (APC/C) ubiquitin ligase activated by its G1 specific adaptor protein Cdh1 is a major regulator of the cell cycle. The APC/C\u003csup\u003eCdh1\u003c/sup\u003e mediates degradation of dozens of proteins, however, the kinetics and requirements for their degradation are largely unknown. We demonstrate that overexpression of the constitutive active \u003ci\u003eCDH1\u003c/i\u003e\u003csup\u003e\u003ci\u003em11\u003c/i\u003e\u003c/sup\u003e mutant that is not inhibited by phosphorylation results in mitotic exit in the absence of the FEAR and MEN pathways, and DNA re-replication in the absence of Cdc7 activity. This mode of mitotic exit also reveals additional requirements for APC/C\u003csup\u003eCdh1\u003c/sup\u003e substrate degradation, which for some substrates such as Pds1 or Clb5 is dephosphorylation, but for others such as Cdc5 is phosphorylation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Phosphorylation_and_dephosphorylation_regulate_APC_C_sup_Cdh1_sup_substrate_degradation/1569452","contentUrl":null,"metadataVersion":6,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-10-08T15:55:10.000Z","registered":"2015-10-08T15:55:11.000Z","published":"2015","updated":"2020-09-04T15:06:48.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.1569452","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}