{"data":{"id":"10.6084/m9.figshare.1569221.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569221.v2","prefix":"10.6084","suffix":"m9.figshare.1569221.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Georis Isabelle","affiliation":[],"nameIdentifiers":[]},{"name":"Tate, Jennifer J","nameType":"Personal","givenName":"Jennifer J","familyName":"Tate","affiliation":[],"nameIdentifiers":[]},{"name":"Vierendeels, Fabienne","nameType":"Personal","givenName":"Fabienne","familyName":"Vierendeels","affiliation":[],"nameIdentifiers":[]},{"name":"Cooper, Terrance G","nameType":"Personal","givenName":"Terrance G","familyName":"Cooper","affiliation":[],"nameIdentifiers":[]},{"name":"Dubois, Evelyne","nameType":"Personal","givenName":"Evelyne","familyName":"Dubois","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Premature termination of \u003ci\u003eGAT1\u003c/i\u003e transcription explains paradoxical negative correlation between nitrogen-responsive mRNA, but constitutive low-level protein production"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"Earth and Environmental Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2016-01-20","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1058476","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1569221","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["817588 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The first step in executing the genetic program of a cell is production of mRNA. In yeast, almost every gene is transcribed as multiple distinct isoforms, differing at their 5′ and/or 3′ termini. However, the implications and functional significance of the transcriptome-wide diversity of mRNA termini remains largely unexplored. In this paper, we show that the \u003ci\u003eGAT1\u003c/i\u003e gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs differing in their 5′ and 3′ termini. Alternative transcription initiation leads to the constitutive, low level production of 2 full length proteins differing in their N-termini, whereas premature transcriptional termination generates a short, highly nitrogen catabolite repression- (NCR-) sensitive transcript that, as far as we can determine, is not translated under the growth conditions we used, but rather likely protects the cell from excess Gat1.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Premature_termination_of_i_GAT1_i_transcription_explains_paradoxical_negative_correlation_between_nitrogen_responsive_mRNA_but_constitutive_low_level_protein_production/1569221/2","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloads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