{"data":{"id":"10.6084/m9.figshare.1569191.v4","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569191.v4","prefix":"10.6084","suffix":"m9.figshare.1569191.v4","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Botbol, Yair","nameType":"Personal","givenName":"Yair","familyName":"Botbol","affiliation":[],"nameIdentifiers":[]},{"name":"Bindi Patel","affiliation":[],"nameIdentifiers":[]},{"name":"Macian, Fernando","nameType":"Personal","givenName":"Fernando","familyName":"Macian","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Common γ-chain cytokine signaling is required for macroautophagy induction during CD4\u003csup\u003e+\u003c/sup\u003e T-cell activation"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Hematology"},{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Meta Science"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-28","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15548627.2015.1089374","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1569191","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["970463 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Macroautophagy is a cellular process that mediates degradation in the lysosome of cytoplasmic components including proteins and organelles. Previous studies have shown that macroautophagy is induced in activated T cells to regulate organelle homeostasis and the cell's energy metabolism. However, the signaling pathways that initiate and regulate activation-induced macroautophagy in T cells have not been identified. Here, we show that activation-induced macroautophagy in T cells depends on signaling from common γ-chain cytokines. Consequently, inhibition of signaling through JAK3, induced downstream of cytokine receptors containing the common γ-chain, prevents full induction of macroautophagy in activated T cells. Moreover, we found that common γ-chain cytokines are not only required for macroautophagy upregulation during T cell activation but can themselves induce macroautophagy. Our data also show that macroautophagy induction in T cells is associated with an increase of LC3 expression that is mediated by a post-transcriptional mechanism. Overall, our findings unveiled a new role for common γ-chain cytokines as a molecular link between autophagy induction and T-cell activation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Common_chain_cytokine_signaling_is_required_for_macroautophagy_induction_during_CD4_sup_sup_T_cell_activation/1569191/4","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"p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