{"data":{"id":"10.6084/m9.figshare.1569191.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569191.v2","prefix":"10.6084","suffix":"m9.figshare.1569191.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Botbol, Yair","nameType":"Personal","givenName":"Yair","familyName":"Botbol","affiliation":[],"nameIdentifiers":[]},{"name":"Bindi Patel","affiliation":[],"nameIdentifiers":[]},{"name":"Macian, Fernando","nameType":"Personal","givenName":"Fernando","familyName":"Macian","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Common γ-chain cytokine signaling is required for macroautophagy induction during CD4\u003csup\u003e+\u003c/sup\u003e T cell activation"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Hematology"},{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Meta Science"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15548627.2015.1089374","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.1569191","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["970463 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Macroautophagy is a cellular process that mediates the degradation in the lysosome of cytoplasmic components including proteins and organelles. Previous studies have shown that macroautophagy is induced in activated T cells to regulate organelle homeostasis and the cell's energy metabolism. However, the signaling pathways that initiate and regulate activation-induced macroautophagy in T cells have not been identified. Herein, we show that activation-induced macroautophagy in T cells depends on signaling from common γ-chain cytokines. Consequently, inhibition of signaling through JAK3, induced downstream of cytokine receptors containing the common γ-chain, prevents full induction of macroautophagy in activated T cells. Moreover, we found that common γ-chain cytokines are not only required for macroautophagy upregulation during T cell activation but can themselves induce macroautophagy. Our data also show that macroautophagy induction in T cells is associated with an increase of LC3 expression that is mediated by a post-transcriptional mechanism. Overall, our findings unveiled a new role for common γ-chain cytokines as a molecular link between autophagy-induction and T cell activation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Common_chain_cytokine_signaling_is_required_for_macroautophagy_induction_during_CD4_sup_sup_T_cell_activation/1569191/2","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citati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