{"data":{"id":"10.6084/m9.figshare.1569161","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569161","prefix":"10.6084","suffix":"m9.figshare.1569161","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Saema, Syed","nameType":"Personal","givenName":"Syed","familyName":"Saema","affiliation":[],"nameIdentifiers":[]},{"name":"Laiq Ur Rahman","affiliation":[],"nameIdentifiers":[]},{"name":"Abhishek Niranjan","affiliation":[],"nameIdentifiers":[]},{"name":"Ahmad, Iffat Zareen","nameType":"Personal","givenName":"Iffat Zareen","familyName":"Ahmad","affiliation":[],"nameIdentifiers":[]},{"name":"Misra, Pratibha","nameType":"Personal","givenName":"Pratibha","familyName":"Misra","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"RNAi-mediated gene silencing of \u003ci\u003eWsSGTL1\u003c/i\u003e in \u003ci\u003eW.somnifera\u003c/i\u003e affects growth and glycosylation pattern"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Developmental Biology"},{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"60506 Virology","subjectScheme":"FOR"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2019-06-03","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15592324.2015.1078064","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["10017586 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Sterol glycosyltransferases (SGTs) belong to family 1 of glycosyltransferases (GTs) and are enzymes responsible for synthesis of sterol–glucosides (SGs) in many organisms. \u003ci\u003eWsSGTL1\u003c/i\u003e is a SGT of \u003ci\u003eWithania somnifera\u003c/i\u003e that has been found associated with plasma membranes. However its biological function in \u003ci\u003eW.somnifera\u003c/i\u003e is largely unknown. In the present study, we have demonstrated through RNAi silencing of \u003ci\u003eWsSGTL1\u003c/i\u003e gene that it performs glycosylation of withanolides and sterols resulting in glycowithanolides and glycosylated sterols respectively, and affects the growth and development of transgenic \u003ci\u003eW.somnifera\u003c/i\u003e. For this, RNAi construct (pFGC1008-\u003ci\u003eWsSGTL1\u003c/i\u003e) was made and genetic transformation was done by \u003ci\u003eAgrobacterium tumefaciens\u003c/i\u003e. HPLC analysis depicts the reduction of withanoside V (the glycowithanolide of \u003ci\u003eW.somnifera\u003c/i\u003e) and a large increase of withanolides (majorly withaferin A) content. Also, a significant decrease in level of glycosylated sterols has been observed. Hence, the obtained data provides an insight into the biological function of \u003ci\u003eWsSGTL1\u003c/i\u003e gene in \u003ci\u003eW.somnifera\u003c/i\u003e.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjEvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLjE1NjkxNjE8L2lkZW50aWZpZXI+CiAgPGNyZWF0b3JzPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5TeWVkIFNhZW1hPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+TGFpcSB1ciBSYWhtYW48L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5BYmhpc2hlayBOaXJhbmphbjwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPklmZmF0IFphcmVlbiBBaG1hZDwvY3JlYXRvck5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlByYXRpYmhhIE1pc3JhPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICA8L2NyZWF0b3JzPgogIDx0aXRsZXM+CiAgICA8dGl0bGU+Uk5BaS1tZWRpYXRlZCBnZW5lIHNpbGVuY2luZyBvZiAmbHQ7aSZndDtXc1NHVEwxJmx0Oy9pJmd0OyBpbiAmbHQ7aSZndDtXLnNvbW5pZmVyYSZsdDsvaSZndDsgYWZmZWN0cyBncm93dGggYW5kIGdseWNvc3lsYXRpb24gcGF0dGVybjwvdGl0bGU+CiAgPC90aXRsZXM+CiAgPGRlc2NyaXB0aW9ucz4KICAgIDxkZXNjcmlwdGlvbiBkZXNjcmlwdGlvblR5cGU9IkFic3RyYWN0Ij4mbHQ7cCZndDtTdGVyb2wgZ2x5Y29zeWx0cmFuc2ZlcmFzZXMgKFNHVHMpIGJlbG9uZyB0byBmYW1pbHkgMSBvZiBnbHljb3N5bHRyYW5zZmVyYXNlcyAoR1RzKSBhbmQgYXJlIGVuenltZXMgcmVzcG9uc2libGUgZm9yIHN5bnRoZXNpcyBvZiBzdGVyb2zigJNnbHVjb3NpZGVzIChTR3MpIGluIG1hbnkgb3JnYW5pc21zLiAmbHQ7aSZndDtXc1NHVEwxJmx0Oy9pJmd0OyBpcyBhIFNHVCBvZiAmbHQ7aSZndDtXaXRoYW5pYSBzb21uaWZlcmEmbHQ7L2kmZ3Q7IHRoYXQgaGFzIGJlZW4gZm91bmQgYXNzb2NpYXRlZCB3aXRoIHBsYXNtYSBtZW1icmFuZXMuIEhvd2V2ZXIgaXRzIGJpb2xvZ2ljYWwgZnVuY3Rpb24gaW4gJmx0O2kmZ3Q7Vy5zb21uaWZlcmEmbHQ7L2kmZ3Q7IGlzIGxhcmdlbHkgdW5rbm93bi4gSW4gdGhlIHByZXNlbnQgc3R1ZHksIHdlIGhhdmUgZGVtb25zdHJhdGVkIHRocm91Z2ggUk5BaSBzaWxlbmNpbmcgb2YgJmx0O2kmZ3Q7V3NTR1RMMSZsdDsvaSZndDsgZ2VuZSB0aGF0IGl0IHBlcmZvcm1zIGdseWNvc3lsYXRpb24gb2Ygd2l0aGFub2xpZGVzIGFuZCBzdGVyb2xzIHJlc3VsdGluZyBpbiBnbHljb3dpdGhhbm9saWRlcyBhbmQgZ2x5Y29zeWxhdGVkIHN0ZXJvbHMgcmVzcGVjdGl2ZWx5LCBhbmQgYWZmZWN0cyB0aGUgZ3Jvd3RoIGFuZCBkZXZlbG9wbWVudCBvZiB0cmFuc2dlbmljICZsdDtpJmd0O1cuc29tbmlmZXJhJmx0Oy9pJmd0Oy4gRm9yIHRoaXMsIFJOQWkgY29uc3RydWN0IChwRkdDMTAwOC0mbHQ7aSZndDtXc1NHVEwxJmx0Oy9pJmd0Oykgd2FzIG1hZGUgYW5kIGdlbmV0aWMgdHJhbnNmb3JtYXRpb24gd2FzIGRvbmUgYnkgJmx0O2kmZ3Q7QWdyb2JhY3Rlcml1bSB0dW1lZmFjaWVucyZsdDsvaSZndDsuIEhQTEMgYW5hbHlzaXMgZGVwaWN0cyB0aGUgcmVkdWN0aW9uIG9mwqB3aXRoYW5vc2lkZSBWICh0aGUgZ2x5Y293aXRoYW5vbGlkZSBvZiAmbHQ7aSZndDtXLnNvbW5pZmVyYSZsdDsvaSZndDspIGFuZCBhIGxhcmdlIGluY3JlYXNlIG9mIHdpdGhhbm9saWRlcyAobWFqb3JseSB3aXRoYWZlcmluIEEpIGNvbnRlbnQuIEFsc28sIGEgc2lnbmlmaWNhbnQgZGVjcmVhc2UgaW4gbGV2ZWwgb2YgZ2x5Y29zeWxhdGVkIHN0ZXJvbHMgaGFzIGJlZW4gb2JzZXJ2ZWQuIEhlbmNlLCB0aGUgb2J0YWluZWQgZGF0YSBwcm92aWRlcyBhbiBpbnNpZ2h0IGludG8gdGhlIGJpb2xvZ2ljYWwgZnVuY3Rpb24gb2YgJmx0O2kmZ3Q7V3NTR1RMMSZsdDsvaSZndDsgZ2VuZSBpbiAmbHQ7aSZndDtXLnNvbW5pZmVyYSZsdDsvaSZndDsuJmx0Oy9wJmd0OzwvZGVzY3JpcHRpb24+CiAgPC9kZXNjcmlwdGlvbnM+CiAgPHN1YmplY3RzPgogICAgPHN1YmplY3Q+QmlvY2hlbWlzdHJ5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+TWVkaWNpbmU8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5DZWxsIEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5Nb2xlY3VsYXIgQmlvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkRldmVsb3BtZW50YWwgQmlvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0IHNjaGVtZVVSST0iaHR0cDovL3d3dy5hYnMuZ292LmF1L2F1c3N0YXRzL2Fic0AubnNmLzAvNkJCNDI3QUI5Njk2QzIyNUNBMjU3NDE4MDAwNDQ2M0UiIHN1YmplY3RTY2hlbWU9IkZPUiI+MTEwMzA5IEluZmVjdGlvdXMgRGlzZWFzZXM8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5QbGFudCBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Qgc2NoZW1lVVJJPSJodHRwOi8vd3d3LmFicy5nb3YuYXUvYXVzc3RhdHMvYWJzQC5uc2YvMC82QkI0MjdBQjk2OTZDMjI1Q0EyNTc0MTgwMDA0NDYzRSIgc3ViamVjdFNjaGVtZT0iRk9SIj42MDUwNiBWaXJvbG9neTwvc3ViamVjdD4KICA8L3N1YmplY3RzPgogIDxwdWJsaXNoZXI+VGF5bG9yICZhbXA7IEZyYW5jaXM8L3B1Ymxpc2hlcj4KICA8cHVibGljYXRpb25ZZWFyPjIwMTU8L3B1YmxpY2F0aW9uWWVhcj4KICA8ZGF0ZXM+CiAgICA8ZGF0ZSBkYXRlVHlwZT0iQ3JlYXRlZCI+MjAxNS0xMC0wODwvZGF0ZT4KICAgIDxkYXRlIGRhdGVUeXBlPSJVcGRhdGVkIj4yMDE5LTA2LTAzPC9kYXRlPgogIDwvZGF0ZXM+CiAgPHJlc291cmNlVHlwZSByZXNvdXJjZVR5cGVHZW5lcmFsPSJEYXRhc2V0Ij5EYXRhc2V0PC9yZXNvdXJjZVR5cGU+CiAgPHNpemVzPgogICAgPHNpemU+MTAwMTc1ODYgQnl0ZXM8L3NpemU+CiAgPC9zaXplcz4KICA8cmVsYXRlZElkZW50aWZpZXJzPgogICAgPHJlbGF0ZWRJZGVudGlmaWVyIHJlbGF0ZWRJZGVudGlmaWVyVHlwZT0iRE9JIiByZWxhdGlvblR5cGU9IklzU3VwcGxlbWVudFRvIj4xMC4xMDgwLzE1NTkyMzI0LjIwMTUuMTA3ODA2NDwvcmVsYXRlZElkZW50aWZpZXI+CiAgPC9yZWxhdGVkSWRlbnRpZmllcnM+CiAgPHJpZ2h0c0xpc3Q+CiAgICA8cmlnaHRzIHJpZ2h0c1VSST0iaHR0cHM6Ly9jcmVhdGl2ZWNvbW1vbnMub3JnL2xpY2Vuc2VzL2J5LzQuMC8iPkNDIEJZIDQuMDwvcmlnaHRzPgogIDwvcmlnaHRzTGlzdD4KPC9yZXNvdXJjZT4=","url":"https://tandf.figshare.com/articles/dataset/RNAi_mediated_gene_silencing_of_i_WsSGTL1_i_in_i_W_somnifera_i_affects_growth_and_glycosylation_pattern/1569161","contentUrl":null,"metadataVersion":5,"schemaVersi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