{"data":{"id":"10.6084/m9.figshare.1569108","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1569108","prefix":"10.6084","suffix":"m9.figshare.1569108","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Osera, Cecilia","nameType":"Personal","givenName":"Cecilia","familyName":"Osera","affiliation":[],"nameIdentifiers":[]},{"name":"Martindale, Jennifer L","nameType":"Personal","givenName":"Jennifer L","familyName":"Martindale","affiliation":[],"nameIdentifiers":[]},{"name":"Marialaura Amadio","affiliation":[],"nameIdentifiers":[]},{"name":"Jiyoung Kim","affiliation":[],"nameIdentifiers":[]},{"name":"Xiaoling Yang","affiliation":[],"nameIdentifiers":[]},{"name":"Moad, Christopher A","nameType":"Personal","givenName":"Christopher A","familyName":"Moad","affiliation":[],"nameIdentifiers":[]},{"name":"Indig, Fred E","nameType":"Personal","givenName":"Fred E","familyName":"Indig","affiliation":[],"nameIdentifiers":[]},{"name":"Govoni, Stefano","nameType":"Personal","givenName":"Stefano","familyName":"Govoni","affiliation":[],"nameIdentifiers":[]},{"name":"Kotb Abdelmohsen","affiliation":[],"nameIdentifiers":[]},{"name":"Gorospe, Myriam","nameType":"Personal","givenName":"Myriam","familyName":"Gorospe","affiliation":[],"nameIdentifiers":[]},{"name":"Pascale, Alessia","nameType":"Personal","givenName":"Alessia","familyName":"Pascale","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Induction of \u003ci\u003eVEGFA\u003c/i\u003e mRNA translation by CoCl\u003csub\u003e2\u003c/sub\u003e mediated by HuR"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Cancer"},{"subject":"111714 Mental Health","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2015-10-08","dateType":"Created"},{"date":"2016-05-25","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15476286.2015.1085276","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["11729311 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Vascular endothelial growth factor (VEGF) A is a master regulator of neovascularization and angiogenesis. VEGFA is potently induced by hypoxia and by pathological conditions including diabetic retinopathy and tumorigenesis. Fine-tuning of VEGFA expression by different stimuli is important for maintaining tissue vascularization and organ homeostasis. Here, we tested the effect of the hypoxia mimetic cobalt chloride (CoCl\u003csub\u003e2\u003c/sub\u003e) on VEGFA expression in human cervical carcinoma HeLa cells. We found that CoCl\u003csub\u003e2\u003c/sub\u003e increased the levels of \u003ci\u003eVEGFA\u003c/i\u003e mRNA and VEGFA protein without affecting \u003ci\u003eVEGFA\u003c/i\u003e mRNA stability. Biotin pulldown analysis to capture the RNA-binding proteins (RBPs) bound to \u003ci\u003eVEGFA\u003c/i\u003e mRNA followed by mass spectrometry analysis revealed that the RBP HuR [human antigen R, a member of the embryonic lethal abnormal vision (ELAV) family of proteins], interacts with \u003ci\u003eVEGFA\u003c/i\u003e mRNA. \u003ci\u003eVEGFA\u003c/i\u003e mRNA-tagging experiments showed that exposure to CoCl\u003csub\u003e2\u003c/sub\u003e increases the interaction of HuR with \u003ci\u003eVEGFA\u003c/i\u003e mRNA and promoted the colocalization of HuR and the distal part of the \u003ci\u003eVEGFA\u003c/i\u003e 3′-untranslated region (UTR) in the cytoplasm. We propose that under hypoxia-like conditions, HuR enhances \u003ci\u003eVEGFA\u003c/i\u003e mRNA translation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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