{"data":{"id":"10.6084/m9.figshare.1568983.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1568983.v2","prefix":"10.6084","suffix":"m9.figshare.1568983.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Tagliazucchi, Davide","nameType":"Personal","givenName":"Davide","familyName":"Tagliazucchi","affiliation":[],"nameIdentifiers":[]},{"name":"Martini, Serena","nameType":"Personal","givenName":"Serena","familyName":"Martini","affiliation":[],"nameIdentifiers":[]},{"name":"Bellesia, Andrea","nameType":"Personal","givenName":"Andrea","familyName":"Bellesia","affiliation":[],"nameIdentifiers":[]},{"name":"Conte, Angela","nameType":"Personal","givenName":"Angela","familyName":"Conte","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Identification of ACE-inhibitory peptides from \u003ci\u003ePhaseolus vulgaris\u003c/i\u003e after \u003ci\u003ein vitro\u003c/i\u003e gastrointestinal digestion"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Cancer"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Biotechnology"},{"subject":"Pharmacology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-10-16","dateType":"Created"},{"date":"2016-01-20","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.3109/09637486.2015.1088940","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.1568983","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["93696 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The objective of this study was to identify the angiotensin I-converting enzyme (ACE)-inhibitory peptides released from thermally treated \u003ci\u003ePhaseolus vulgaris\u003c/i\u003e (pinto) whole beans after \u003ci\u003ein vitro\u003c/i\u003e gastrointestinal digestion. The degree of hydrolysis increased during digestion reaching a value of 50% at the end of the pancreatic digestion. The \u0026lt;3 kDa fraction of the postpancreatic sample showed high ACE-inhibitory activity (\u003ci\u003eIC\u003c/i\u003e\u003csub\u003e50\u003c/sub\u003e = 105.6 ± 2.1 μg of peptides/mL). Peptides responsible for the ACE-inhibitory activity were isolated by reverse-phase high-performance liquid chromatography (HPLC). Three fractions, showing the highest inhibitory activity, were selected for tandem mass spectrometry (MS/MS) experiments. Eleven of the identified sequences have previously been described as ACE-inhibitors. Most of the identified bioactive peptides have a hydrophobic amino acid, (iso)leucine or phenylalanine, or proline at the C-terminal position, which is crucial for their ACE-inhibitory activity. The sequence of some peptides allowed us to anticipate the presence of ACE-inhibitory activity.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/Identification_of_ACE_inhibitory_peptides_from_i_Phaseolus_vulgaris_i_after_i_in_vitro_i_gastrointestinal_digestion/1568983/2","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"creat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