{"data":{"id":"10.6084/m9.figshare.1568895.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1568895.v1","prefix":"10.6084","suffix":"m9.figshare.1568895.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Tamo Fukamizo","affiliation":[],"nameIdentifiers":[]},{"name":"Chalermchai Somboonpatarakun","affiliation":[],"nameIdentifiers":[]},{"name":"Shinya, Shoko","nameType":"Personal","givenName":"Shoko","familyName":"Shinya","affiliation":[],"nameIdentifiers":[]},{"name":"Kawaguchi, Yuya","nameType":"Personal","givenName":"Yuya","familyName":"Kawaguchi","affiliation":[],"nameIdentifiers":[]},{"name":"Araki, Tomohiro","nameType":"Personal","givenName":"Tomohiro","familyName":"Araki","affiliation":[],"nameIdentifiers":[]},{"name":"Sompong Klaynongsruang","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"A goose-type lysozyme from ostrich (\u003ci\u003eStruthio camelus\u003c/i\u003e) egg white: multiple roles of His101 in its enzymatic reaction"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-10-02","dateType":"Created"},{"date":"2016-01-20","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2015.1091716","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.1568895","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["953551 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"A goose-type lysozyme from ostrich egg white (OEL) was produced by \u003ci\u003eEscherichia coli\u003c/i\u003e expression system, and the role of His101 of OEL in the enzymatic reaction was investigated by NMR spectroscopy, thermal unfolding, and theoretical modeling of the enzymatic hydrolysis of hexa-\u003ci\u003eN\u003c/i\u003e-acetylchitohexaose, (GlcNAc)\u003csub\u003e6\u003c/sub\u003e. Although the binding of tri-\u003ci\u003eN\u003c/i\u003e-acetylchitotriose, (GlcNAc)\u003csub\u003e3\u003c/sub\u003e, to OEL perturbed several backbone resonances in the \u003csup\u003e1\u003c/sup\u003eH–\u003csup\u003e15\u003c/sup\u003eN HSQC spectrum, the chemical shift of the backbone resonance of His101 was not significantly affected. However, apparent p\u003ci\u003eK\u003c/i\u003e\u003csub\u003ea\u003c/sub\u003e values of His101 and Lys102 determined from the pH titration curves of the backbone chemical shifts were markedly shifted by (GlcNAc)\u003csub\u003e3\u003c/sub\u003e binding. Thermal unfolding experiments and modeling study of (GlcNAc)\u003csub\u003e6\u003c/sub\u003e hydrolysis using a His101-mutated OEL (H101A-OEL) revealed that the His101 mutation affected not only sugar residue affinities at subsites −3 and −2 but also the rate constant for bond cleavage. His101 appears to play multiple roles in the substrate binding and the catalytic reaction.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/A_goose_type_lysozyme_from_ostrich_i_Struthio_camelus_i_egg_white_multiple_roles_of_His101_in_its_enzymatic_reaction/1568895/1","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state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