{"data":{"id":"10.6084/m9.figshare.1568818.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.1568818.v1","prefix":"10.6084","suffix":"m9.figshare.1568818.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"R. Sowdhamini","affiliation":[],"nameIdentifiers":[]},{"name":"Pritha Ghosh","affiliation":[],"nameIdentifiers":[]},{"name":"Grellscheid, Sushma Nagaraja","nameType":"Personal","givenName":"Sushma Nagaraja","familyName":"Grellscheid","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"A Tale of Two Paralogs: Human Transformer2 proteins with differential RNA-binding affinities"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"110309 Infectious Diseases","subjectScheme":"FOR"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Health sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Evolutionary Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Physics"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-09-26","dateType":"Created"},{"date":"2016-01-20","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.1568818","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["149269667 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The Transformer2 (Tra2) proteins in humans are homologues of the \u003ci\u003eDrosophila\u003c/i\u003e Tra2 protein. One of the two RNA-binding paralogs, Tra2β, has been very well-studied over the past decade, but not much is known about Tra2α. It was very recently shown that the two proteins demonstrate the phenomenon of paralog compensation. Here, we provide a structural basis for this genetic backup circuit, using molecular modelling and dynamics studies. We show that the two proteins display similar binding specificities, but differential affinities to a short GAA-rich RNA stretch. Starting from the 6-nucleotide RNA in the solution structure, close to4000 virtual mutations were modelled on RNA and the domain-RNA interactionswere studied after energy minimization to convergence. Separately, anotherknown 13-nucleotide stretch was docked and the domain-RNA interactionswere observed through a 100-ns dynamics trajectory. We have also demonstrated the ‘compensatory’ mechanism at the level of domains in one of the domain repeat-containing RNA-binding proteins.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/articles/dataset/A_Tale_of_Two_Paralogs_Human_Transformer2_proteins_with_differential_RNA_binding_affinities/1568818/1","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-20T07:17:34.000Z","registered":"2016-01-20T07:17:35.000Z","published":"2015","u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